We narrowed to 860 results for: PLE

...ever like to write about a recent plasmid deposit please sign up here.        A retroviral mouse CRISPR...Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the promoter.  ...should serve as both a powerful tool for generating complex regulatory systems and further the study of natural...

... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...collective priority list. There has been progress—for example, a number of funders (e.g., the NIH) now look for...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then...

... enzymes, some of which are also functional in multiple species (read Arbor Biotechnologies' press release...nt direct repeat) mediating potent knockdown. Multiplexing from the CRISPR array yielded >90% knockdown... necessary. Konermann et al. compared CasRx to multiple RNA regulating methods: small hairpin RNA interference...

...For safety and production reasons, multiple plasmids with many components are used to package lab grown...with each other to allow DNA synthesis of the complementary strand to yield dsDNA. Unlike LTRs, ITRs are...): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: ...

...researchers new to GCE aren’t able to successfully complete their experiments and many others are dubious ...universal techniques that researchers can easily implement in their own labs. They provide resources, knowledge...bulletin board on their website specifically so people can talk about GCE. In fact, their first protocol...

...cool because they can transmit information, for example, from one of the 180 regions to another or from...even further defined population of neurons. For example, say I wanted to access all the neurons that signaled... Multiple Authors...

...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...targeting an exon in your gene of interest, for example. Some customization is required, but many viral...them an ideal delivery system for human patients. Multiple viral-based gene therapies have already been approved...

...specific region of your protein of interest. For example, if you are studying a protein that may bind a ...recognize the protein even though it is present in your sample. Additionally, there is a lower likelihood that...higher than that of polyclonal antibodies. For example, it takes longer to develop a hybridoma than it...

... look at how we create this content, using the example of our Antibody Guide, which was published alongside...can. We even have a Slack channel just for asking people if they can write or review things and another ...require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few ...

...of both AsLOV2 and the nanobody. As a proof-of-principle the Toettcher Lab monitored how AsLOV2 insertion...manipulation of the signaling pathway.  As a proof-of-principle, the team created an OptoNB to control the Ras...levers to pull to control signaling pathways. For example, if the OptoNB could bind and block a receptor ...

...agriculture, are now synonymous with GM to the point that people cannot imagine any use for GM technology other ... is not food, not medicine, and not mandatory – people can decide for themselves if they want this beautiful...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...

...preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce non-physiological...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...levels seen in HHCS patients. These are just three examples of how RaPID can be used to study RNA-protein ...

...based on size. Whether higher molecular weight complexes are detected will depend on the homodimer affinity...will be problem-free. In figure 1 we provide an example of the OSER assay with a number of fluorescent ...tested. To conclude, we recommend that you try a couple of different fluorescent proteins when protein ...

... practice to compare degradation rates between samples treated with or without an autophagy inhibitor....accumulates in the vacuole. See Figure 2 for an example of what fluorescence looks like for cytoplasmic...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...

... the LoxP sites, which is more difficult than a simple knock out. Looking for some permanent knock out...transcripts. When transiently introduced, they can deplete endogenous protein levels for 1-3 days. There’... tunable control These systems can be easily implemented for exogenously introduced proteins, as many ...

...ever like to write about a recent plasmid deposit please sign up here.        AAV delivery of split base... Type IIS restriction sites to easily assemble multiple DNA fragments. This system has been used to construct...controlled by Cre recombination. Each cell can have multiple copies of the transgene that recombine independently...

... further demonstrated that PRIME-del can be “multiplexed” using pooled paired pegRNAs.  Read the paper...Xue’s lab also uses paired pegRNAs targeting complementary DNA strands to program large genomic deletions... without small insertions. But, instead, PEDAR couples this deletion process to a prime editing enzyme...

...intensive to process a large number of microbiome samples. To enable large-scale, longitudinal studies of...and can approximate mRNA concentration within a sample. Using this system, the Collins lab was able to...lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it ...

...affect it. While tumors may be the stars, there's plenty of action outside of tumor cells, and recent studies...deeply connected to these 3 stages and contains multiple cell types: fibroblasts, myofibroblasts, neuroendocrine...spread and drug resistance? Although we’ve seen how multiple types of cells affect tumor progression, enzymes...

...below) Because off-target sequences have less complementarity to the gRNA, they have a higher propensity ...for their on-target and off-target activity at multiple genomic locations in HEK cells. Further testing... Hiroshi, et al. "Crystal structure of Cas9 in complex with guide RNA and target DNA." Cell 156.5 (2014...