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We narrowed to 847 results for: PLE

Showing: 621 - 640 of 847 results

  1. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...of both AsLOV2 and the nanobody. As a proof-of-principle the Toettcher Lab monitored how AsLOV2 insertion...manipulation of the signaling pathway.  As a proof-of-principle, the team created an OptoNB to control the Ras...levers to pull to control signaling pathways. For example, if the OptoNB could bind and block a receptor ...

  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...agriculture, are now synonymous with GM to the point that people cannot imagine any use for GM technology other ... is not food, not medicine, and not mandatory – people can decide for themselves if they want this beautiful...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...

  3. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ... the LoxP sites, which is more difficult than a simple knock out. Looking for some permanent knock out...transcripts. When transiently introduced, they can deplete endogenous protein levels for 1-3 days. There’... tunable control These systems can be easily implemented for exogenously introduced proteins, as many ...

  4. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce non-physiological...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...levels seen in HHCS patients. These are just three examples of how RaPID can be used to study RNA-protein ...

  5. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ... further demonstrated that PRIME-del can be “multiplexed” using pooled paired pegRNAs.  Read the paper...Xue’s lab also uses paired pegRNAs targeting complementary DNA strands to program large genomic deletions... without small insertions. But, instead, PEDAR couples this deletion process to a prime editing enzyme...

  6. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...based on size. Whether higher molecular weight complexes are detected will depend on the homodimer affinity...will be problem-free. In figure 1 we provide an example of the OSER assay with a number of fluorescent ...tested. To conclude, we recommend that you try a couple of different fluorescent proteins when protein ...

  7. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ... practice to compare degradation rates between samples treated with or without an autophagy inhibitor....accumulates in the vacuole. See Figure 2 for an example of what fluorescence looks like for cytoplasmic...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...

  8. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...intensive to process a large number of microbiome samples. To enable large-scale, longitudinal studies of...and can approximate mRNA concentration within a sample. Using this system, the Collins lab was able to...lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it ...

  9. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here.        AAV delivery of split base... Type IIS restriction sites to easily assemble multiple DNA fragments. This system has been used to construct...controlled by Cre recombination. Each cell can have multiple copies of the transgene that recombine independently...

  10. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...affect it. While tumors may be the stars, there's plenty of action outside of tumor cells, and recent studies...deeply connected to these 3 stages and contains multiple cell types: fibroblasts, myofibroblasts, neuroendocrine...spread and drug resistance? Although we’ve seen how multiple types of cells affect tumor progression, enzymes...

  11. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ...below) Because off-target sequences have less complementarity to the gRNA, they have a higher propensity ...for their on-target and off-target activity at multiple genomic locations in HEK cells. Further testing... Hiroshi, et al. "Crystal structure of Cas9 in complex with guide RNA and target DNA." Cell 156.5 (2014...

  12. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...erlotinib. Looking at barcode abundances among multiple replicate populations, they found that similar...went on to show that combination treatment with multiple drugs reveals even smaller preexisting populations...dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine 21.5 (2015): 440-...

  13. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...have all heard. It’s a truth that extends to both people and the tools we use. Scientists utilize an array... to stop functioning properly or be something completely different than what was originally intended. ...Addgene receives deposited plasmids, they undergo complete plasmid sequencing using an in-house Illumina ...

  14. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...achieved with a 30-minute incubation, even with multiplexed guides. Figure 1: A) Cell-penetrating ...generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which they named... Multiple Authors...

  15. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...animal models of disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore...current delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked... Multiple Authors...

  16. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...viruses that can infect people, cells, and animals. Some of these viruses are completely inert, but others ...protection are key to lab safety and the same principles apply to viral handling. Gloves are always required...

  17. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in this ... well-characterized Cas enzymes, which rely on multiple active sites. In addition, Casɸ has a minimal ...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth...

  18. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...poster should catch the audience’s eye and draw people in. That can be accomplished with an exciting title...clearly identified and interpreted in less than a couple of minutes. How does one accomplish all of that...column left-to-right, which is the natural way that people expect to read. The key to the success of this ...

  19. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...others are studying and gives you a sense of what people are excited about. Following scientists in fields...more about authors by exploring author pages (for example, Melina Fan’s author page). These pages show all... Key features Setting up a personalized feed is simple. You can enter as many search terms as you like...

  20. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...anything. Viruses are complicated and they perform complex biological tasks, both of which are factors in ...Ugai et al., 2002). If the virus will be used in a couple of days, some researchers prefer to store freshly...: 16510845. 4. Krajden, Mel, et al. "Effect of multiple freeze-thaw cycles on hepatitis B virus DNA and...
Showing: 621 - 640 of 847 results