We narrowed to 837 results for: RING
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TypeBlog PostPublishedApril 20, 2023, 4 p.m.... could present something coherent or to quickly string together all the words that I could remember related...conferences, or even just provide quick updates during a company meeting increases visibility of your ...
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The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m....them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up as to whether it’s faster...cloning method Find blog posts about scientific sharing here Download the Plasmids 101 eBook Resources... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific symposium...inspiration to resolve the technical hurtle of monitoring protein turnover of specific proteins in situ... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....rather blunt instrument for controlling CRISPR. To bring greater control to Acr activity as a biological ...benthamiana or Arabidopsis thaliana (Xing et al., 2016)). During Agrobacterium-mediated DNA transfer, each GOI is... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m.... a musician, then metastasize is touring. But you know what a touring musician needs? A backstage team... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog PostPublishedOct. 1, 2019, 1:21 p.m....you on LinkedIn, you will lose opportunities and hiring managers will think it is odd. For those planning...any of these people, including and especially the hiring manager. This is considered intrusive and it’s ... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether... and the concentration of the protein solution. During the experiment, the solution is diluted, complicating... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....purification preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce...events that occur on a short timescale, such as during drug action or cell signaling. Limitations of RaPID... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....as cereblon (CRBN) or von Hippel-Lindau (VHL) to bring the endogenous degradation machinery into close ...lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal fusions and... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m....informing potential for success in a patient-mutation bearing knock-in mouse model for CTD. Congratulations the...questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building upon... -
Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....similar to a Fab fragment but created through engineering instead of cleavage. They are comprised of the...stable up to 80°C and can refold correctly after denaturing. Designed ankyrin repeat proteins, or DARPins... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....enough that there is virtually no chance of it occurring randomly. Therefore, inserting loxP sites at deliberate...are active only in certain cell or tissue types, during certain stages of development, or by making the... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....spread. They can be inherited by daughter cells during cellular reproduction, similar to the host’s chromosome...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria to form... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m....options are available to you, it may be worth considering an antibody raised in a different species. ... strategically plan primary/secondary antibody pairing such that each target has a unique species or isotype... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....resulted altogether in 40 new ABE8 variants. When comparing on-target DNA editing efficiency in a human cell...and ABE7.10. They quantified genomic editing by measuring the reduction in protein expression of these six... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog PostPublishedNov. 7, 2017, 1:59 p.m....hold great promise for improving or potentially curing human diseases that were previously thought to ...as gene delivery tools: Unlike most naturally occurring viruses used in clinical trials, wild type AAV... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m....CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability ...Nanoblades editing efficiencies of primary cells. Comparing Nanoblades to Existing CRISPR Delivery Methods... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog PostPublishedJune 26, 2018, 1:33 p.m....features that allow such “chemical rescue” in vivo. Engineering the human disease alleles into cells for testing...might consider prescribing GdnHCl to patients suffering from rare diseases arising from Arg mutations.... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....specific locations and give rise to progenitors during growth and development or that blood flows through.... While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these sequences... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog PostPublishedSept. 13, 2016, 2:30 p.m.... of Research (FoR) symposia held across the USA during this time have revealed that young scientists are...Biochemistry and Molecular Biology/ FoR Hack Day during which several projects were initiated. Recent developments...