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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 842 results for: RING

Showing: 621 - 640 of 842 results

  1. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ... ChIP is an incredibly useful application for exploring these interactions, and can give valuable insight...and the Three Steps (Crosslinking, Fragmenting, Capturing), if you ask me.  Each of the three steps need...

  2. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light...  Mary Gearing...

  3. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ... called the Resource Identification Initiative. During this project, authors are asked to add an identifier...guidelines for the reporting of laboratory animals during in vivo research and the CARE guidelines for case...

  4. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...however, they tend to be unstable and may be lost during budding. Yeast Centromere plasmids (YCp): These...with yeast, due to high rates of spontaneously occurring resistant mutants and the insensitivity of yeast...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...spectral properties of the fluorophores you are considering to make sure that they’ll be compatible with ...target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores. We also touched on fluorophores...

  6. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ... could present something coherent or to quickly string together all the words that I could remember related...conferences, or even just provide quick updates during a company meeting increases visibility of your ...

  7. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up as to whether it’s faster...cloning method Find blog posts about scientific sharing here Download the Plasmids 101 eBook Resources...

  8. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether... and the concentration of the protein solution. During the experiment, the solution is diluted, complicating...

  9. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...purification preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce...events that occur on a short timescale, such as during drug action or cell signaling. Limitations of RaPID...

  10. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...as cereblon (CRBN) or von Hippel-Lindau (VHL) to bring the endogenous degradation machinery into close ...lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal fusions and...

  11. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...you on LinkedIn, you will lose opportunities and hiring managers will think it is odd. For those planning...any of these people, including and especially the hiring manager. This is considered intrusive and it’s ...

  13. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...informing potential for success in a patient-mutation bearing knock-in mouse model for CTD. Congratulations the...questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building upon...

  14. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...similar to a Fab fragment but created through engineering instead of cleavage. They are comprised of the...stable up to 80°C and can refold correctly after denaturing.  Designed ankyrin repeat proteins, or DARPins...

  15. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific symposium...inspiration to resolve the technical hurtle of monitoring protein turnover of specific proteins in situ...

  17. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...enough that there is virtually no chance of it occurring randomly. Therefore, inserting loxP sites at deliberate...are active only in certain cell or tissue types, during certain stages of development, or by making the...

  18. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...spread. They can be inherited by daughter cells during cellular reproduction, similar to the host’s chromosome...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria to form...

  19. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...options are available to you, it may be worth considering an antibody raised in a different species.    ... strategically plan primary/secondary antibody pairing such that each target has a unique species or isotype...
Showing: 621 - 640 of 842 results