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  1. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...sonification. Though it helps capture weaker or more transient DNA-protein interactions, the reliance on fixation...McKay lab we study how differential access to transcriptional regulatory elements is controlled during development...

  2. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...includes an antibiotic resistance marker. After transformation, plate the cells on medium containing the corresponding...selection will select for cells that have been transformed with the plasmid backbone (with or without your...

  3. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...from the DNA. These apurinic sites if in double stranded DNA are repaired by base excision repair, but ...but can lead to mutations in single stranded DNA undergoing replication.   DNase on the other hand can digest...

  4. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...of University of Michigan says, “Addgene has transformed the way in which we share plasmids and other ...shipping logistics and facilitate all material transfer agreements, thereby freeing up time for our depositors...

  5. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...some proteins. Protein folding issues, steric hindrances, and destabilization can be brought about by ...DOI: 10.1038/521274a Arribere, J. A., et al., Translation readthrough mitigation. Nature. (2016) 534, 719...

  7. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...joyful that getting to the final step and the transformed bacteria refuse to grow. Eventual success was...

  8. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ... for control elements such as promoters and transcription terminators, the boundaries are less obvious...to newly sequenced strains through our mismatch tolerant detection algorithm and thereby speed up the annotation...

  11. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...mRFP-GFP-LC3 Tandem Fluorescent Protein, but following translation, this fusion protein is cleaved to generate one...phosphatidylethanolamine (PE) and recruited to autophagasomal membranes, which leads to its degradation by autophagy. ...

  13. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...brain science, my curiosity did not ultimately translate to a desire to do the research itself. Several...past and chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry...

  14. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...  For example, while “PCR test” has recently transitioned from jargon to non-jargon in the context of ...number of characteristics. Common ones include age range, familiarity with the subject, shared interests,...

  15. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...programmed regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests...Science journalism programs at various universities range from week-long workshops to more in-depth master...

  16. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need to increase...sequencing. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million...

  17. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...used to generate inversions, deletions, and translocations. It also provides researchers with many different...Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely new career...

  18. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...play a role in many cell processes involving cell membrane dynamics. When they learned there was a disease...desire to understand the early events in the transformation of healthy cells into melanoma cells. They’...

  19. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...can generate a fluorescent protein fusion, transcriptional reporter, and loss-of-function allele in just...markers. Bob Goldstein’s lab previously used a transgene selection strategy for their C. elegans/CRISPR...

  20. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...on a range of topics including biotech and research tools, computing performance, and randomized control...
Showing: 621 - 640 of 796 results