Skip to main content
Addgene
Showing: 621 - 640 of 803 results

  1. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...inside and outside your field. Your colleagues, lab mates, and PI can provide feedback on your technical presentation...pressed for time, limit yourself to five to ten minutes per poster. Ask the presenter to give the “elevator...

  2. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...(Can I attend this conference?) and with my lab mates on which lab jobs needed to be done (Who is responsible...your needs actually are: Maybe you only need 15 minutes of the two hour time slot, so she can take the ...

  3. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ... compared to other systems because the system modulates protein post-translationally, unlike many other...through interacting ligands. After an E3 ligase facilitates ubiquitination of the Halo-tagged protein in ...

  4. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...also enabled imaging of their propagation along dendrites for the first time (Figure 3). The team also used...a patient with non-neurodegenerative diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024...

  5. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...applied to PhD programs in Biology around the United States in 2021, I know that this process only became even...program you’ll be using for the interview. Having notes close to your computer with things you want to highlight...

  7. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...eliminate every cancerous cell. Even so, to pose the greatest threat, cancerous cells still need to successfully...the research community has access to all of the latest resources? In response to this challenge, Addgene...

  9. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...sequence. Co-delivery of wild-type Cas9 and a gRNA generates a double-strand break in the target DNA, which...experiment, the use of appropriate controls, multiple replicates and several cell types can strengthen your results...

  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...using a pinhole.  However, the excitation light excites all of the fluorophores as it passes through the...the laser light sheet, typically 2-6  microns, illuminates only one thin plane of the sample surrounding...

  11. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...pathogens to their hosts. I spoke with her about what motivates her research, the MoClo Kit she and Sylvestre ...of terminators than are commonly used as we have tested them to show that they work well in plants and ...

  12. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...Aligning two nucleotide sequences is probably the fastest BLAST search to perform and will save you time ...comparing different isoforms. In addition to our favorites, there are a number of sequence alignment tools...

  13. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...study by the NSF showed that among US 4th graders tested on the Science National Assessment of Educational... you don’t have children but are interested in testing your SciComm skills or are interested in a career...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...results in preclinical studies some potential candidates for rare to ultra-rare ocular diseases are being... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

  15. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...longer educational videos, and Twitter, for sharing updates, new deposits, and new blog posts!    How to... Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication should be...

  16. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban, MD. Figure 3 depicts the ... the National Academy of Sciences of the United States of America, 54(1), 137–144. https://doi.org/10.1073...

  17. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...within the folded protein (green region). Methanol dehydrates the protein, which can disrupt protein structure...at your antibody datasheet to see if it has been tested in IF and if a protocol is available. When possible...

  19. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...checked circle. Typically, a yellow check mark indicates that a particular parameter was automatically ...sequencing bases downstream of the cut. Summary ICE generates NGS-quality CRISPR editing analysis from Sanger...

  20. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...for labs and nonprofits, who tend to prioritize candidates that are excited about their work and mission... will be close to that of a professional email. Notes from real-life hiring managers In preparation for...
Showing: 621 - 640 of 803 results