We narrowed to 867 results for: TOR
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TypeBlog PostPublishedApril 8, 2024, 2:15 p.m....two options: “Search Repository” and “Deposit Materials”. The “Search Repository” function is our same...section: Tools of the Day. Our plasmid and viral vector of the day have a fresh new look and a fresh new...
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Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m....Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley,...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to... blog posts Resources at Addgene Browse the repository Find educational resources at Addgene Search ... -
Top 10 Open Science Developments of 2013
TypeBlog PostPublishedDec. 19, 2013, 3:46 p.m...., even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...preference to journal access granted via a public repository or authors’ own web sites. 9) The year 2013... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog PostPublishedMay 7, 2019, 12:39 p.m....MCF10A cellular models. Ba/F3 and MCF10A are growth factor dependent cell lines and any mutations that that...advantages will be revealed in the absence of growth factor. The team assigned the mutations to functional ...significantly increased cell viability, and these “activators” may be the direct drivers of tumor formation... -
9 Tips to Achieve Success in Academia
TypeBlog PostPublishedApril 1, 2014, 5:33 p.m...., and young Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages...different ways to succeed – as loner or gregarious; dictator or laissez-faire; theory or practice; business-like... -
We're Updating Our Hold for Publication Policy
TypeBlog PostPublishedJune 24, 2025, 1:15 p.m....original policy was straightforward, but cumbersome: depositors had to email us with permission to release their... are left in the hold status and reach out to depositors about them, but we all know how easy it is to...your work. Plus, it’s costly for us to process and store materials that aren’t distributed or for our teams... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog PostPublishedJune 10, 2025, 1:15 p.m....affiliated TniQ protein recruits transposition cofactors. The transposon proteins integrate a payload sequence... Figure 1: Mechanism of CAST systems. A Cas effector (Cascade in Type I systems; Cas12k in Type V-K...Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase. Science... -
An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m.... gene in a pLenti vector backbone. During those five weeks, I worked with my mentors, Katherine, Amrita...exchanged contact with Amy C., Addgene’s intern coordinator shortly after hearing her talk about Addgene’... -
Build Your CRISPR Vocabulary
TypeBlog PostPublishedJuly 2, 2024, 1:15 p.m....used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...array: The section of the bacterial chromosome that stores a genetic record of previously encountered foreign...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m....neurons that trigger the CPG (central pattern generator) (Wyart et al., 2009). The CPG is a neural circuit... involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...optogenetics blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog PostPublishedJune 29, 2023, 7 p.m....stuffing new depositor welcome kits, note taking for protocol video production, and keeping inventory of our... the people at Addgene have become my friends, mentors, and some of my favorite people to talk to. This... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog PostPublishedJune 19, 2018, 1:38 p.m....biodistribution therefore introduce additional confounding factors when attempting to interpret signals from these...collapse E. coli re-express arg1 gas vesicles and restore their ultrasound signal. Using acoustic reporter...years to clinically treat enteric infection and inflammatory bowel conditions. In these experiments, arg1... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog PostPublishedNov. 1, 2017, 2:04 p.m....department.” Caitlin does this by acting as a facilitator between her team, the sales department, and operations...business.” Caitlin’s advice for budding science communicators: “My recommendation would be to take any opportunity...education tools? Check out this Addgene podcast with educator and science rap mastermind Tom McFadden Get tips... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog PostPublishedOct. 3, 2017, 12:11 p.m....For this series, I’ll interview three science communicators who work in the biotech, education, or nonprofit...job. “I am really more of a multimedia science communicator than a science writer these days. I do some ... to talk to other scientists when I am in new territory. Perhaps there are examples of science writers... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....cannot produce them. By using yeast integration vectors (see for example this yeast cloning kit) she intends...expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant Cry proteins... develop technologies to protect fresh water repositories in Mexico. At Addgene we're happy to be able... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m....individuals through stock or investor pay outs. Addgene has no investors or stock. All of our proceeds...proceeds go into running the repository and serving scientists better. That includes keeping fees as low as ... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog PostPublishedMay 18, 2017, 2:30 p.m....biological sensors’) are biological tools that monitor a process or detect a given molecule. The sensor...in. Depending on the process you would like to monitor, there may be multiple steps you could examine,...biosensors. These constructs are often used to monitor enzymatic activity or phosphorylation over time... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m....match sequence information contributed by the depositor, published backbone sequences, and any known sequences...analyze sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps...experiments like restriction digests. We’ll have a tutorial showing all of the features available on our Snapgene... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m....patient partners so that they can help tell their stories. I was paired with a six-year-old boy named AJ ...caused by a single R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate bone regeneration...where my wife Lianna has made her career as Senior Director of Biology, is helping us fight against FOP in... -
10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m....you questions. Remember that people like a good story and science involves characters – in this case you...or other places online, and consider how those stories are being told. Twitter can be a good way to follow...sharing – both your plasmids and your research stories. Consider practicing communicating your research...