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We narrowed to 856 results for: TOR

Showing: 621 - 640 of 856 results

  1. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biological sensors’) are biological tools that monitor a process or detect a given molecule. The sensor...in. Depending on the process you would like to monitor, there may be multiple steps you could examine,...biosensors. These constructs are often used to monitor enzymatic activity or phosphorylation over time...

  2. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...stuffing new depositor welcome kits, note taking for protocol video production, and keeping inventory of our... the people at Addgene have become my friends, mentors, and some of my favorite people to talk to. This...

  3. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...original policy was straightforward, but cumbersome: depositors had to email us with permission to release their... are left in the hold status and reach out to depositors about them, but we all know how easy it is to...your work. Plus, it’s costly for us to process and store materials that aren’t distributed or for our teams...

  4. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...affiliated TniQ protein recruits transposition cofactors. The transposon proteins integrate a payload sequence... Figure 1: Mechanism of CAST systems. A Cas effector (Cascade in Type I systems; Cas12k in Type V-K...Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase. Science...

  5. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ... gene in a pLenti vector backbone. During those five weeks, I worked with my mentors, Katherine, Amrita...exchanged contact with Amy C., Addgene’s intern coordinator shortly after hearing her talk about Addgene’...

  6. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...array: The section of the bacterial chromosome that stores a genetic record of previously encountered foreign...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

  7. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ...department.” Caitlin does this by acting as a facilitator between her team, the sales department, and operations...business.” Caitlin’s advice for budding science communicators: “My recommendation would be to take any opportunity...education tools? Check out this Addgene podcast with educator and science rap mastermind Tom McFadden Get tips...

  8. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    Published
    Oct. 3, 2017, 12:11 p.m.
    ...For this series, I’ll interview three science communicators who work in the biotech, education, or nonprofit...job. “I am really more of a multimedia science communicator than a science writer these days. I do some ... to talk to other scientists when I am in new territory. Perhaps there are examples of science writers...

  9. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...patient partners so that they can help tell their stories. I was paired with a six-year-old boy named AJ ...caused by a single R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate bone regeneration...where my wife Lianna has made her career as Senior Director of Biology, is helping us fight against FOP in...

  10. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...you questions. Remember that people like a good story and science involves characters – in this case you...or other places online, and consider how those stories are being told. Twitter can be a good way to follow...sharing – both your plasmids and your research stories. Consider practicing communicating your research...

  11. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...2018 PubMed PMID:29109157. Nanobody toolkit to monitor retrograde transport at the cell surface Article...able to bind fluorescent proteins derived from A. victoria the functionalized nanobodies described here can...

  12. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...meaningful day job only came into focus when an English tutor noted my eclectic mix of science and arts papers...venturing forth from tertiary study, the myriad trajectories ahead can be condensed into two discernible ...truly benefit others. Herein, a good science communicator—with an understanding of how to appeal to a ...

  13. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...research materials, such as plasmids and viral vectors. However, it typically takes a few years for a .... Daniela Bourges, Addgene’s interim Executive Director. “NABOR provides a new model for the creation ... the NIH’s BRAIN Initiative has been funding laboratories, such as Dr. Trimmer’s lab, to create recombinant...

  14. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...will describe how to copy a cDNA from one vector into a new vector that is better suited for analyzing the...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...

  15. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    .... I had just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge and knew...outside of academia, a job as a ‘Cancer Marketing Coordinator’ came up at Abcam. Unlike other job descriptions...knew there would be a way around this. First off I tore up my “Scientific CV” and wrote a more business ...

  16. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...who may not be experts, can you give me a brief history of optogenetics? How long has this idea been around...optogenetic tools for neuroscience back in 2005, both activators as well as silencers of neural activity. Since...brain is very complex and we've hosted hundreds of visitors in our lab to help them understand their specific...

  17. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...great deal more information that one might like to store about FPs than is currently represented at fpvis...a centralized, community-editable resource for storage and dissemination of information about FPs. FPbase.org...elaborate spectrum analysis tools, and a better way to store and represent within-study photobleaching comparisons...

  18. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...classification system, including its history, biology, and usages in the lab. History In 1884, while studying infected...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...

  19. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination in cultured...control the CRISPR system activity.  Using an AAV vector may help with size limitations. In addition, non-viral...

  20. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think we missed...expression of the tumor-seeking CAR (chimeric antigen receptor), whereas a ubiquitous promoter drives expression...can be made for expressing your favorite T-cell receptor, or perhaps any other tool that can benefit from...
Showing: 621 - 640 of 856 results