We narrowed to 643 results for: VARS

1. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...karyotypically normal and are able to differentiate into various cell types of all three germ layers [7]. To further...

2. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   Published

   Oct. 19, 2017, 1:54 p.m.

   ...use of fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre...

3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

   Type

   Blog Post

   Published

   Sept. 21, 2017, 2:07 p.m.

   ...focus on what makes this molecule unique, and the various applications of Cas13a. The origins of Cas13a:...

4. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...for biosensors. Aptamer biosensors are used in a variety of applications, including detection of environmental...

5. How to Write a Scientific Review Article

   Type

   Blog Post

   Published

   Feb. 16, 2017, 3:30 p.m.

   ... class of proteins was mutated, as reported in various studies. We made a table that listed each protein...

6. Antibiotic Resistance: An Old Solution but a New Problem

   Type

   Blog Post

   Published

   Nov. 10, 2016, 3:30 p.m.

   ...organism. As for multi-drug resistance: definitions vary in the literature, but generally organisms with ...

7. Healthcare Consulting: A Door to the Business of Life Sciences

   Type

   Blog Post

   Published

   Oct. 13, 2016, 2:30 p.m.

   ... quickly learn, and apply their knowledge to a variety of problems. In this post, I’ll share what I’ve...

8. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   Published

   Oct. 1, 2015, 2:30 p.m.

   ...brain region of interest could be low or highly variable without the behavioral test. Another readout may...

9. CRISPR 101: Validating Your Genome Edit

   Type

   Blog Post

   Published

   Nov. 3, 2022, 12:15 p.m.

   ...Pinello L. CRISPRitz: rapid, high-throughput and variant-aware in silico off-target site identification ...

10. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...basic approaches to conflict resolution based on various levels of assertiveness and cooperation: avoiding...

11. Plasmids 101: Biotinylation

    Type

    Blog Post

    Published

    Nov. 15, 2018, 1:50 p.m.

    ...chemicals (biotinylation reagents). There are a variety of biotinylation reagents available that differ...

12. Antibodies 101: Introduction to Immunofluorescence

    Type

    Blog Post

    Published

    Nov. 16, 2021, 2:15 p.m.

    ...produce and distribute antibodies validate them in a variety of applications, including IF (or IHC or ICC), ...

13. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type

    Blog Post

    Published

    July 10, 2018, 1:08 p.m.

    ... avoid scattering intellectual property across various software providers used to supplement their paper...

14. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ...the Addgene Blog Learn about High Fidelity Cas9 Variants How to Design Your gRNA Delivering CRISPR Ribonucleoproteins...

15. March for Science

    Type

    Blog Post

    Published

    April 21, 2017, 2:30 p.m.

    ...her PhD from the Systems Biology department at Harvard University. She is a scientist with a passion for...

16. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ... temperature and length of induction, which will vary between different proteins. Beyond these standard...

17. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ...diseases, the mutational landscape is broad and varied, necessitating the development of many distinct...

18. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...equal. Some work better than others. There are a variety of resources online to guide you in gRNA design...

19. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    Published

    Dec. 17, 2020, 1:30 p.m.

    ...being used in any genetic background, but require variable amounts of experimental manipulation and screening...

20. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include investigating...