We narrowed to 872 results for: fer

... of interest). Mesophilic microbes are highly preferable (and successful) as host systems since their ...to guess. Think about it like winter tires in different conditions: the more flexible rubber of winter...reagent repositories and the myriad facilities offered by them which help foster a more productive and...

...explaining more of her role, which eventually led me to transfer to her team and begin my product management (PM... part of my job is getting to talk to so many different people! I communicate with customers, software... get software product management internships, preferably on teams that run within the agile framework ...

... ideas come from social media conversations, conferences, and questions our scientific support staff receives...line with Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer ...constantly reviewing the resources we currently offer to identify gaps and add to them as techniques evolve...

...experiment moving. Happy editing! References and Resources References Hendel, A., Bak, R., Clark, J. et...used in CRISPR-Cas9 experiments – the “gRNA” – suffers from the same issues as your experimental RNA. ...

...’s classrooms,” BioBuilder answers the call by offering a variety of educational programs all focused ...opened in August 2017.  This flexible teaching space offers STEM programming, workshops, and customized programs...point into further STEM exploration.  Further offerings for both STEM teachers and STEM-loving students...

...can lead to variable expression levels due to differences in copy number.  And there are the usual transfection...Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection...multiplexing, leave them in the comments below.        References 1. Zetsche et al. "Multiplex Gene Editing by ...

...synthesis, and multifragment assembly methods are preferred. No such restriction applies to deletions; using...common methods for site directed mutagenesis   References 1. Trehan, Ashutosh, et al. (2016). “REPLACR-... on Addgene.org Browse Our Molecular Biology Reference Pages Choose Your Perfect Plasmid Backbone Find...

...expression, PTGs will likely be adaptable to many different biological systems. Although Xie et. al characterized...in plants, PTGs will likely work well in many different biological systems. Size is also a factor; the...technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex editing capability...

...difficult for an individual lab to produce - Hybridomas offer an alternative and renewable source of monoclonal... their use. Each RANbody is detectable by two different tags: a reporter and an HA tag. The reporter comes... link to its sequence in the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-...

...overexpressed from a plasmid but overexpression may interfere with the system. Another method for studying protein...whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization...

...affect protein binding patterns. Hereditary Hyperferritinemia-Cataract Syndrome (HHCS), a disease characterized...the Iron Response Element (IRE) RNA motif of the ferritin light chain RNA transcript. IRE motifs are bound...The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, D.S., Neela...

...newly independent faculty member studying sex differences in thoracic aortic disease,” Prakash says. In..., the lab hopes to capture the signalosome of different receptors upon stimulation. “To study cellular... Jennifer Tsang...

...bispecific, meaning that each scFv can target a different antigen; binding of each can be either simultaneous... a scientist looking for that perfect fit!  References and Resources Additional resources on the Addgene...AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018). Drug targeting and ...

...dTomato) that promote interactions between the FPs interfere with native interactions, preventing proper localization...papers should be used with caution, since the difference between studies is substantial (e.g, the poor...conclude, we recommend that you try a couple of different fluorescent proteins when protein tagging is the...

...mutations). Although the precise mutations were different than those found in eSpCas9, the effect on specificity...and/or therapeutic settings. Will combining the different mutations found in each of these great tools further...continued improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered...

...replicates would have different labels - i.e. they would stochastically arise from different cells throughout...development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al. "Studying clonal...

...because it “causes recombination” (although later referred to as the “cyclization recombinase”), is a 38 ...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination...

...base or 4-base overhangs, and with a variety of different enzymes and cycling conditions (see Table 1). ... cycling 3-base  PaqCl®, 1x T4 DNA Ligase Buffer 37-16 °C cycling 4-base PaqCl, 1x NEBridge... and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera, R. B., Langhorst...

...dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... like the genetic cargo in one plasmid (the transfer plasmid), the envelope (Env) gene on a separate plasmid...Env, Gag, and Pol genes are not encoded in the transfer plasmid, the component that physically integrates...

...updated in 2023 by Susanna Stroik.  References and Resources References Yuxuan Wu et al. "Correction of a...frequency isn’t high enough to make a physiological difference, then the treatment will not work in a meaningful...