We narrowed to 829 results for: lars

...in open science this year. Here it goes, in no particular order, my top 10 open science developments, 2013...-respected and high-profile universities. We particularly recommend Eric Lander's intro to biology course...

...programs are discussed below). Chpater 3: SITN Popularity and Events - Amy discusses some of the programs...programs SITN runs and describes some of the more popular topics from the Science by the Pint series. Chapter...

... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that...

...functionality. Fluorescent proteins One of the largest plasmid collections deposited with Addgene is the...expression, as well as a number of fusions for cellular localization studies including mEmerald-Mito-7...

...strategies for using combinations of AAV-based molecular tools for targeted neuronal manipulation. The ...of interest: Cell-type specific expression of molecular tools and sensors Combinations of classical genetic...

...a network of researchers who may be working on similar projects and should make it easier for them to ...missing an important collection or if you think a particular plasmid or type of plasmid is missing from our...

...facilitating the live study of chromatin dynamics at a particular locus. A limitation of this system is the single-color...studies of cell cycle progression, epigenetics and cellular reactions to external stimuli. It could also have...

...Cre-lox recombination is an incredibly useful molecular biology tool, but like any biological system, ... yeast recombinases: KD (from Kluyveromyces drosophilarum), R (from Zygosaccharomyces rouxii), B2 (from...

...dimerization of HLA B27, involved in the onset of spondylarthritis, using Bioluminescence Resonance Energy Transfer...the University of California, specialising in Molecular Biology. She has worked in a range of labs - cancer...

... nm will pass through animal and human tissues largely unimpeded. The challenge then was to develop fluorescent... Ultrasound wavelengths, on the other hand, are larger than the size of a cell, so they scatter much less...

... small deletions, while H840A is biased towards large insertions. These profiles are likely due to the... Oligonucleotides) Use 100 nt homology arms for large insertions (long ssDNA; IDT Megamer® ssDNA Fragments...

...writer  When Hans was finishing up his PhD in Molecular Neuroscience at the University of Iowa, he was...over hosting them. Since then, webinars make up a larger part of Hans’ workload. “I am currently getting...

...overlapping PAM. In both cases, GeoCas9 and SpyCas had similar levels of editing. See figure 4 in Harrington et...thermostability could also be used with in vitro molecular bio applications that require high temperature...

...day at Addgene last summer, through the La Vida Scholars summer program. I was unsure whether or not I ...which was the impression I got from the La Vida Scholars tour. After some time had passed, I reached out...

... or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small (Rep52 and Rep40) Rep...Targeting and Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248...

...purification, it will be expressed along with all other cellular proteins within the expression system (bacteria...protein) with amylose resin. The MBP tag, although large, can actually increase expression and solubility...

...internal control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

...essential cofactors for many tools used in modern molecular biology like restriction enzymes and polymerases...posts Resources on Addgene.org Check out the Molecular Biology Reference Find protocols on plasmid cloning...

..., as well as to optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an...fluorescent protein plasmids at Addgene Read our Molecular Biology Reference ...

...Protein Science at the University of Connecticut. Molecular cloning is the isolation, insertion and amplification... recombinant DNA without sequence alteration. Molecular cloning techniques are some of the most widely...