We narrowed to 796 results for: lat
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  TypeBlog PostPublishedJune 18, 2019, 1:15 p.m....provides improved detection for tracking large populations of neurons in wide-field imaging (Dana et al.... intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV, as well as...
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  Celebrate National Book Month with AddgeneTypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music: how we perceive...roles microbes play in our ecosystem, examining relationships between microbes and insects, mammals, and plants...
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  Summer SciComm Series: A PhD in Science CommunicationTypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....the same myths and misconceptions fifteen years later. What does that look like? One of my more recent...designs could work to slow the spread of illness. I later helped run workshops where I showed people how they...
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  New Neuroscience Tool: The iGluSnFR3 Glutamate SensorTypeBlog PostPublishedApril 7, 2022, 1:15 p.m....trigger other forms of neuronal signaling and regulation. To create iGluSnFR, the Looger lab wedged GFP...soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: ...
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  Four Factors that Differentiate the Stem Cell FieldTypeBlog PostPublishedSept. 28, 2016, 2:35 p.m....that stem cell scientists are so focused on translational research is encouraging. Patients are key to...depositors Marius Wernig, Kristin Baldwin, Kathrin Plath, Hans Schöler, and closing keynote speaker Rudolph...
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  Optimizing Donor DNA for Enhanced CRISPR Genome EditingTypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....process in detail often suggests new strategies to manipulate that process. We began our research with a simple...approaches. Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed...
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  Choosing Your Fluorescent Proteins for Multi-Color ImagingTypeBlog PostPublishedOct. 9, 2014, 3 p.m....is a challenge. To help keep you abreast of the latest fluorescent proteins, I maintain an interactive... is sufficient to use a spectrum for a closely related protein, if you know that your protein of interest...
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  Advice for Scientists Starting a LabTypeBlog PostPublishedApril 29, 2014, 1:43 p.m....pub with us in the evening, wanting to know our latest results as soon as the gels ran.” Dr. Ellis tries...emphasis on genetic engineering, sequencing, and translation of results to companies.” Take home message Starting...
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  Quick Guide to Working with Drosophila Part 3: Genome Engineering in FliesTypeBlog PostPublishedJuly 28, 2017, 1:30 p.m.... determine the implications of your genetic manipulations. You can produce some very beautiful immunofluorescent...model organism is in its amenability to genetic manipulation. This is just a primer so that you are aware...
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  Luminescent Imaging with Nano-lanternsTypeBlog PostPublishedMay 25, 2017, 2:30 p.m....continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki...signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used a linear...
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  New and Upcoming Viral Vectors - May 2020TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....offered by the system that allows for specific manipulation of neurons. We have expanded our AAV collection...Chemogenetics offers noninvasive methods for manipulating cell activity, particularly in the brain, and...
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  The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate ItTypeBlog PostPublishedJan. 30, 2020, 2:15 p.m....more credible than they actually are. Throwing out plates that don’t respond as expected, use of representative...different results. Different setting, different population, new genetic variation, or different handling...
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  Antibodies 101: IsotypesTypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....region length. Now for some essential isotype nomenclature. Antibody heavy chain proteins as well as the...start by co-expressing IgM and IgD antibodies, but later in an immune response, the variable region of an...
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  Behind the scenes: Addgene’s new search engine and moreTypeBlog PostPublishedDec. 12, 2019, 2:22 p.m....adding more terms. Weighting We determined the relative importance of different bits of information for...shortcuts to specific catalog items as well as related items (for example, a kit that contains a plasmid...
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  Plasmids 101: Screening Strategies Used in Plasmid CloningTypeBlog PostPublishedSept. 15, 2020, 1:15 p.m....information (more on this below). Plating the transformation on ampicillin plates containing X-gal allows you...
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  Viral Vectors 101: Inverted Terminal RepeatsTypeBlog PostPublishedOct. 19, 2023, 1:15 p.m....in the native virus. Since the WT sequence is relatively unstable, a more stable 130 bp version is used... D. V. (2023). AAV- based vector improvements unrelated to capsid protein modification. Front Med, 10,...
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  Dos & Don'ts When Publishing a Scientific ManuscriptTypeBlog PostPublishedMay 22, 2014, 4:02 p.m....? Sometimes, the funders of your research may stipulate that your article must be published in an open...data behind the figures and results. This is a relatively new development, and it allows reviewers and ...
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  Click Biology: How to Click and Play to Explore BiologyTypeBlog PostPublishedOct. 21, 2025, 1:15 p.m....homology-directed repair efficiency by linking the repair template VirD2 HUH-tag For creating DNA-protein conjugates... are many convenient tools out there, not just relating to proteins but also covalent reactions to DNA...
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  Addgene: The First Twenty YearsTypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....the Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative. We also...shipped out our two millionth item, and had a cumulative 120,000 citations of individual items in our ...
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  dTAG - You're it!TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation of FKBP12F36V-BRD4...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ...