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...option for the experiment above, but it’s also important not to load yourself up with too many tasks. If...ll discuss in the planning section below, it’s important to dedicate specific amounts of time to specific...

...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good... score their probability of off-target events: CRISPOR, Cas-OFFinder, CCTop, and many more. While such...

...protocol is a good place to get started.  One important consideration when deciding whether you can do...specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies from...

...system to modify your target DNA, you first electroporate a linear donor DNA substrate (either dsDNA or...Expression of lambda red recombination genes. Electroporation of substrate DNA and outgrowth of bacteria....

...a pressurized buffer called sheath fluid and transported through tubes or capillaries to a laser. As the...tag your protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside...

...refer to Cong et al. (2013) for more details. One important note is that this ‘split RNA’ design is not as.... (2013). One-Step generation of mice carrying reporter and conditional alleles by CRISPR/CAS-Mediated...

...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...

...access to IPI educational resources developed to support use of their antibody tools. Addgene also offers...

...series! Follow @addgene on TikTok for bite-sized portions of antibody concepts. Each short video answers...

...connect with other researchers working with AAV, support open science, and maybe even win a $500 travel ...

...and vote for your favorite! Our mascot who has supported us over the past eight years now has a new friend...

...Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors...

... students and teachers interested in JEI (the importance of word of mouth and personal networking!) and...

...working with Andrew, the head of our scientific support team, to tackle any experimental questions you ...

...trick When combining reagents in the lab, it is important to mix them thoroughly before use.  For efficient...

...experiences. It will contain all of the juicy and important details that you won’t find in the final publication...

...introduced us to RUBY-red, a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh...

...Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038/s41598-017-02693-1. PubMed PMID...

...background and education 3:49-4:35 : Kingdom on opportunities to go into science in Malawi 4:36-5:46 : How...

...18265393. Additional Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great ...