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We narrowed to 816 results for: LAS

Showing: 321 - 330 of 816 results

  1. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...Allen Institute for Cell Science Plasmid Collection Check Out Plasmids for FRET Check Out the Fluorescent..., most secreted proteins are produced in the endoplasmid reticulum, modified in the golgi and then transported... on the gene names in the figure below to find plasmids containing that particular gene. ... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers Institute...Prescott (Monash University). You can find more plasmids for labeling your subcellular structure of interest...interest in the Allen Institute for Cell Science Plasmid Collection. You can also use the library of full-length...Localization Poster here! References 1. Allen Institute Plasmid Pages 2. Cooper GM. 2000. The Cell: A Molecular...

  2. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ....  This is in contrast to confocal laser microscopy where the laser and the reflected light travel through...Voie, David Burns and Francis Spelman focused a laser beam into a thin sheet to illuminate a fluorescent...than the modern cameras used in LSFM. In LSFM the laser light sheet, typically 2-6  microns, illuminates...-bleaching or photo-damage than in conventional laser scanning microscopy . Since image acquisition is...Ultramicroscope.  In addition, adjusting the thickness of the laser sheet and using multi-view imaging can provide enhanced...compared to transgenic animals (17).  These viral plasmids are available through Addgene.  The combination...University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an...

  3. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...Using arabidopsis in the classroom Resources on Addgene.org Find plant plasmids Visit our plant resource...this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium tumeficans - a...available (Weigel and Glazebrook, 2002). Find plant plasmid resources at Addgene  Choosing an expression system...options, but here are a couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway...prefer Golden Gate assembly, there are a series of plasmids from the Dinneny lab that additionally allow for... plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are both great options for this, depending...culture you are growing definitely contains your plasmid. Grow lots of Agrobacterium: The more agrobacterium...

  4. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ... His solution? Bringing his research into the classroom. I had the opportunity to talk with Provost recently...interest. The result was a positive experience for the class but one that left him overwhelmed. Therefore, he...measured activity, MDH is particularly amenable to in-class labs. Provost ultimately settled on a two-semester...critical thinking skills (Knutson et al., 2010a,b). Classroom-based research projects have also been proposed...professional success (many of my students include their in-class research experience on their resumes). CUREs are... sounds like an exciting opportunity for your classroom, I highly recommend you try implementing one. ...on planarian flatworms both in and out of the classroom.   References and Resources References Bangera...

  5. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids 101: Green Fluorescent Protein Resources...Fluorescent Protein Collection Find Plasmid Kits at Addgene Find Plasmid Cloning Protocols References 1....combination of different genetic sequences into plasmid DNA. In high throughput cloning techniques, although...MXS chaining MXS-chaining was designed to create plasmids for fluorescence imaging or flow cytometry applications...modules (Figure 2) are contained in individual plasmids and are each flanked by the same multiple cloning...throughput cloning is to facilitate the construction of plasmids with many components. The creators of the MXS-... plasmid cloning...

  6. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...enChIP Plasmids? All Hodaka Fujii lab plasmids Sign up to receive email alerts when new plasmids from ... shopping for a wide range of plasmids. The merit of depositing plasmids to Addgene has been spectacular...this technology and its advantages? Fujii: In the last several years, my lab has been working on development... researchers all over the world have requested plasmids for enChIP through Addgene. Retroviral Expression...user-friendly and precise. We will deposit the plasmids of these systems to Addgene in the near future...Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's Advisory...

  7. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs...countries enriched our repository with 15,020 new plasmids — a testament to the scientific community's commitment...next-generation sequencing, setting new standards for plasmid quality control. Through our partnership with the... viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged on demand...toward giving you more choice in how you receive plasmids — as DNA or bacterial stabs — ‌and reinventing...sequences and characterization data for over 150,000 plasmids, empowering bioinformaticians, data scientists...developers to unlock the potential of our extensive plasmid database — because we believe that innovation flourishes...

  8. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ... about the last 10 years @Addgene Check out our impressive stats from 10 Years of Plasmid Sharing Or ... into the successful, internationally renowned plasmid repository that it is today. Although I wish I ... founders, Melina Fan, Ken Fan, and Benjie Chen. Last week, the Addgene 10-year Anniversary Gala celebrated...music playing all night was hand-picked (like our plasmid samples!) by Addgenies. If you were there, you ...video highlighting the changes at Addgene over the last 10 years as well as the many people that make up...Addgene uses E. coli bacteria to generate copies of plasmids. The community of scientists, part of the Addgene... complicated process of requesting and sharing plasmids has become simplified through Addgene. Looking...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ....org Check out Our Hot Plasmids Articles to See Compiled Info on New Plasmids Review the CRISPR Field...beginning. I also recommend using the citation style of (Last name, Year) in the document while writing, because...style to whatever the journal requires. Using the (Last name, Year) format also has the benefit of exposing..., we summarized how frequently each member of a class of proteins was mutated, as reported in various ...We made a table that listed each protein in the class, then for each protein we listed all the studies... we made a schematic of all the proteins in the class that showed the relative sizes and the conserved...similarities and differences among proteins in the class. Offer your perspective It doesn’t have to be long...

  10. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...with a resident endoplasmic reticulum (ER) protein instead localizes to the cytoplasm? Or more troubling...other than the cytoplasm. Generally, FPs evolved in or were engineered for use in cytoplasmic environments...mitochondria and chloroplasts, the extracellular milieu, and gram negative bacteria periplasm. Efforts to ...FPs) localized to compartments other than the cytoplasm. That is, unfortunately, some investigators realize...quality control of secretory proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik...mediate mitotic inheritance independently of the endoplasmic reticulum." The Journal of cell biology 154.2...
Showing: 321 - 330 of 816 results