...accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs...countries enriched our repository with 15,020 new plasmids — a testament to the scientific community's commitment...next-generation sequencing, setting new standards for plasmid quality control. Through our partnership with the... viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged on demand...toward giving you more choice in how you receive plasmids — as DNA or bacterial stabs — ‌and reinventing...sequences and characterization data for over 150,000 plasmids, empowering bioinformaticians, data scientists...developers to unlock the potential of our extensive plasmid database — because we believe that innovation flourishes...

... about the last 10 years @Addgene Check out our impressive stats from 10 Years of Plasmid Sharing Or ... into the successful, internationally renowned plasmid repository that it is today. Although I wish I ... founders, Melina Fan, Ken Fan, and Benjie Chen. Last week, the Addgene 10-year Anniversary Gala celebrated...music playing all night was hand-picked (like our plasmid samples!) by Addgenies. If you were there, you ...video highlighting the changes at Addgene over the last 10 years as well as the many people that make up...Addgene uses E. coli bacteria to generate copies of plasmids. The community of scientists, part of the Addgene... complicated process of requesting and sharing plasmids has become simplified through Addgene. Looking...

....org Check out Our Hot Plasmids Articles to See Compiled Info on New Plasmids Review the CRISPR Field...beginning. I also recommend using the citation style of (Last name, Year) in the document while writing, because...style to whatever the journal requires. Using the (Last name, Year) format also has the benefit of exposing..., we summarized how frequently each member of a class of proteins was mutated, as reported in various ...We made a table that listed each protein in the class, then for each protein we listed all the studies... we made a schematic of all the proteins in the class that showed the relative sizes and the conserved...similarities and differences among proteins in the class. Offer your perspective It doesn’t have to be long...

...with a resident endoplasmic reticulum (ER) protein instead localizes to the cytoplasm? Or more troubling...other than the cytoplasm. Generally, FPs evolved in or were engineered for use in cytoplasmic environments...mitochondria and chloroplasts, the extracellular milieu, and gram negative bacteria periplasm. Efforts to ...FPs) localized to compartments other than the cytoplasm. That is, unfortunately, some investigators realize...quality control of secretory proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik...mediate mitotic inheritance independently of the endoplasmic reticulum." The Journal of cell biology 154.2...

...that they called a Glycosylase Base Editor, or GBE, for its reliance on the glycosylase UNG to crease abasic...Converts C → G; also called a glycosylase base editor (GBE). GBE Glycosylase Base Editor A base editor ...Uracil DNA N-Glycosylase Removes uracil from DNA, creating an abasic site. UGI Uracil Glycosylase Inhibitor...at its original job. They removed the uracil glycosylase inhibitor (UGI) components, which inhibit BER...instead introduced its opposite, a uracil DNA N-glycosylase (UNG) from E. coli. The resulting construct, ..., Rosser, S. J., Bi, C., & Zhang, X. (2021). Glycosylase base editors enable C-to-A and C-to-G base changes...Additional resources on Addgene.org Browse CRISPR plasmids for base editing Get a broad overview with our...

...More than a model System - Arabidopsis in the classroom With funding from the American Society of Plant... the kits, known collectively as Greening the Classroom, were developed and tested by ABRC staff. These...an ordering account. Using Arabidopsis in the classroom has many benefits. Engaging students in active...science curriculum. Growing Arabidopsis in the classroom does not require a huge investment in resources.... Another benefit of using Arabidopsis in the classroom is that staff from the ABRC are here to support... people in science learning. Whether visiting classrooms, hosting field trips, conducting teacher workshops...resource or you want to introduce Arabidopsis to your class for the first time – we want to hear from you. Reach...

...Biotechnology which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen....with other fluorescent proteins. Find mNeonGreen plasmids here! mTFP1: a monomeric teal fluorescent protein...492 nm, respectively), they gave this a teal classification rather than cyan and named the protein mTFP1...physiologically relevant changes in pH. Find mTFP1 plasmids here! mWasabi: a bright green fluorescent protein...excite at 400 nm while EGFP does. Find mWasabi plasmids here! One of the most notable things about these...are best suited for certain studies. If you have plasmids containing mNeonGreen, mTFP1, or mWasabi that ...with the scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "...

...About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts  Suggest Plasmid Tools for the ...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...to changing pH, and they can rupture E. coli spheroplasts to release encapsulated proteins. Video by ACS...these fluorescent R bodies can rupture E. coli spheroplasts (cells which have had their cell walls removed...produced R-bodies that could extend at different pHs. Plasmids expressing expressing both wild type and mutant...acssynbio.5b00237. PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni, ...

...the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source...useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase itself, please ...genome fully intact, while, with a standard DNA plasmid transfection, a double-stranded break may occur...by utilizing a piggyBac™ transposon in a donor plasmid carrying the DNA edit of interest, along with a...piggyBac™ transposase enzyme [4]. By using a donor plasmid containing a negative selection marker such as ...kill off any cells that still contain the donor plasmid after excision by PBx, thereby leaving behind only...cytotoxicity [5]. Second, instead of using a donor-plasmid, you could use a single-stranded oligodeoxynucleotide...

...Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio Plasmids ... of “bringing tomorrow’s science into today’s classrooms,” BioBuilder answers the call by offering a variety...forget the technical details they hear in their classes,” she said, “but they remember the moments when..., Kuldell also knew that the best days in the classroom are characterized by intense student engagement...and how — some choose to run full semester-long classes, some pick and choose a few lessons to include ...book chapter and an illustrated, downloadable classroom poster — with both of these resources road tested...any stage of completion, from pitch to prototype. Last year’s BioBuilderClub teams’ biological engineering...