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  1. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...and Maria have lots of ideas and are happy to speculate, they know they can’t answer these questions alone...have collected a variety of data that might help formulate some hypotheses. They are excited to see other...

  2. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...universities!) This guide tells you how to use online platforms, such as Zoom, to determine if a program is the... yours does and then use that time! Different platforms have different quirks, so remember to practice...

  3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...     The requirement for Cas9 transcription and translation delays editing What are Cas9-gRNA ribonucleoproteins... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...

  4. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...communities and into species not previously cultured or isolated (Rubin et al., 2020).  Figure 3: Multiplexed...-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR...

  5. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...the hard work for you by immunizing an alpaca, isolating HCab antibodies from its serum, and meticulously...rapid epitope-mapping and native protein complex isolation. eLife. PubMed PMID: 26633879 PubMed Central PMCID...

  6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...easiest switch from fluorescent proteins given the relative simplicity of the system and the availability ...PMC2802578. 4. Xue, Lin, et al. "Imaging and manipulating proteins in live cells through covalent labeling...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...expression, shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not...applications where a distinct subset of the cellular population needs to be targeted. Q6: Are transfer plasmids...

  8. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...feasible, it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged...for Cas9. However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs...

  9. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...therapeutic drugs, vinblastine and vincristine, are regulated in the medicinal plant, the Catharanthus roseus...investigate transcription factor candidates and the regulation of genes in the biosynthetic pathway. Why plant...

  10. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ... of the Z pathway" is vague and "what proteins regulate the Z pathway?" is an open-ended list of proteins...proteins. Instead, consider "how do proteins X and Y regulate the Z pathway?" A well-defined outcome makes it...

  11. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...session is to start with something completely unrelated to the issue and consider how it could be shaped...problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for...

  12. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...and experimental needs. Since its invention in the late 1990s, Gateway cloning technology has become very...Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR to Quickly...

  13. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...to repair the DNA using the edited strand as a template — in this case, encouraging it to incorporate ...Like other transversion editors, this editor had relatively high indel rates ranging from 4.7% to 30% at ...

  14. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...; the importance of which will be elaborated on later. As for what job fairs to consider, some of the ...the way. Make connections, and nurture those relationships. Then when you are ready to apply for your dream...

  15. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...different opinions, and some of them would acknowledge later that without my “neutral” presence to alleviate ... that nobody is really owning. Conflict in this latter scenario usually manifests as the assumption “I...

  16. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular... Originally reported as a monomer16, IFP2.0 was later found to be a dimer3,15. ND-not determined in parallel...

  17. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...applications, they have become indispensable tools for manipulating cells and organisms. They can be used for a ...This is because most titering methods rely on isolating genomic DNA from transduced cells and quantifying...

  18. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ... require sequencing of many samples at once for later comparison. These next-generation sequencing techniques...technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move ...

  20. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...replaces a target region with a newly synthesized, templated sequence. And the story still isn’t over! Keep...Base Editor A base editor that relies on DNA glycosylation to create an abasic site. In this post, it refers...
Showing: 641 - 660 of 781 results