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...enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal ...size and reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its affinity...

...membrane-impermeant and can only enter cells with compromised plasma membranes — i.e., dead cells — while live...which yields a much weaker signal on live cells compared to dead cells. As a result, the largely negative...

...undetectable in the blood. But the virus doesn’t completely disappear; it just hides in latently infected...viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple...

...enhancer regions (Mendenhall et al., 2013). By comparing gene activation when enhancers were active or ...increased gene activation at the targeted site compared to other Tet1 delivery systems. Transcriptional...

... domain structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG...). Figure 5: TurboCas protein labeling compared to immunoprecipitation. The top panel depicts ...

... cell types. Figure 1: Diagram of AAV components. Created with BioRender.com. AAV vectors ...Transduction of Airway Epithelial Cells in Mouse Lungs Compared to That of AAV2 Vectors. Journal of Virology, ...

...direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates which reduces...worms are perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted...

...sites are both recognized by Cre, but they are comprised of different sequences (loxp and lox2272) and ...essential that every new vector, whether it be a completely new transgene or merely a new lot, be thoroughly...

...experiences! These applications can be highly competitive, so asking for help from mentors and other students... Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences of First-Generation...

...parameter to consider is the contrast ratio- a comparison of PA-FP brightness before and after photoactivation...variants are more monomeric than mEos2 (mEos4b is completely monomeric) and display bright and photostable...

...vitro Both in vitro and in vivo Epigenetic marks Complete removal to convert to iPSCs and re-establishment...in science communication and publishing after completing her postdoctoral training from Massachusetts ...

...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

...That's why we gave our written protocol a video companion! Follow along as Addgenie Meghan and Abi run a...

...bioinformatics quest, which led us to Seqera Labs, a company that provides open source workflow orchestration...

...vectors, but what we lacked were videos guiding completely new members of the lab or those taking introductory...

...well as alternatives to Cas9. All of this is accompanied by new or refreshed figures made using BioRender.com...

...updating our CRISPR guide. Our CRISPR guide is a comprehensive resource that covers most things CRISPR - from...

... gave to me: efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth...

...Keystone Meeting Eric presented a poster with data comparing Addgene global deposit and distribution trends...

...Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with NEB's DADExperts: Matthew...