We narrowed to 817 results for: LARS
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TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....challenge in the best of cases. But it can be particularly challenging when your only antibody option is...al., 2013). One issue with this strategy is that large complexes may not permeate through your tissue samples...
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Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....This is, for the intrepid researcher looking for molecular biology tools, a very useful trait. Virus structure... Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered by the... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m.... tissue-specific promoter sounds simpler, this modular system saves you from having to create a new fly...and overexpress viral genes. Additionally, other modular expression systems exist (eg. LexA/LexAop and Cre... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m....conferences/meetings over and over to get to know the regulars (or better yet, become a volunteer). Naturally...stay in touch with all of their connections on a regular basis. It is also not at all necessary. Once you... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog PostPublishedJune 30, 2020, 1:15 p.m....sessions, not your walks between sessions Like many large conferences with multiple sessions happening at ...walking up to the mic and asking your question in a large conference room puts butterflies in your stomach... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m....central We’re working on a project where we aim to do large scale DNA preps with multiple samples without cross...time and space in your own lab, you should give similar workshops a try if you’re ever looking to try out... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....greater science literacy and education have more polarized beliefs on controversial science topics. Proceedings..., L. L., Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived... -
DAP arrays for multiplex gene editing
TypeBlog PostPublishedJuly 7, 2022, 1:15 p.m....prime editing, achieving up to 3-loci MPE with similar high efficiencies, which is scalable for a higher...genes against multiple genetic diseases, such as muscular dystrophies, heart disease, and type 2 diabetes... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....are being given in vivo, it is difficult to use larger enzymes. To reduce the impact of off-target mutations...B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular physiology. https://doi.org/10.1002/jcp.30064... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....sensor to the membrane and displays it on the extracellular side. In addition to the original PDGFR TMD,...promoter, FLP-dependent expression GFAP: Glial fibrillary acidic protein (promoter). Good for Glial expression... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....would take years and millions, if not billions, of dollars, but by approaching it from a new direction, we...Science Communications Team at QBI UCSF. They are particularly interested in facilitating collaborative science... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog PostPublishedFeb. 13, 2024, 2:15 p.m....Monoclonal antibody drugs are popular therapeutics for a plethora of disease conditions, from cancer ...Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis: From Manufacturing to Preclinical Studies. ... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....evolutionarily it evolved much slower and bears more similarity to the ancient yeast strains and metazoan cells...Additional Resources on the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors ... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m.... names have a B, J, X, or Z. Some alternative, similar apps: Findings Lab Notebook Cell Culture Split...Protoctols for Your Research Catch Up On Your Molecular Biology Basics ... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m.... dialogue and community among participants, clarification - new and useful information, and invigoration...meetings. One-on-Ones: Managers should set up regular check-ins with their employees. 15-30 minutes weekly... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m....light-based control of signaling pathways. This is particularly exciting because using light to control signaling...With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....post-transcriptional gene expression across the majority of cellular pathways. Despite several years of research, the...pushed the field further with the creation of the largest miRNA sensor collection reported to date, containing... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m.... this process, you will first need to do some molecular cloning. Depending on whether you are trying to...send your plasmid to a company. For a few hundred dollars, they will inject the plasmid into fly embryos ... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog PostPublishedJuly 6, 2017, 1:59 p.m....immunosurveillance. The presented study analysed a model of hepatocellular carcinoma, a cancer which can develop from ...27913448. 3. Cai, En, et al. "Visualizing dynamic microvillar search and stabilization during ligand detection... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m.... Prevent Interruptions If you need to focus, a large set of headphones can act as a Do Not Disturb sign...would build a wall of pipette tip boxes when she particularly needed to focus, which was extremely effective...