Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 795 results for: LAT;

Showing: 641 - 660 of 795 results

  1. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...the requirements of the specific assay. For the latter three, secondary antibodies are indicated when ...absolute quantitative analysis of a sample. Instead, relative quantification can be done using either the direct...

  2. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...always easy to track at the transcriptional or translational level. However, several genetic tools have been.... These allow for not only recording but also modulating bioelectric states in vivo. For example, the ...

  5. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system takes the...Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable marker; attB site...

  6. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...correcting monogenetic diseases. Now, almost 10 years later, CRISPR’s potential for treatment is no longer an...CRISPR to disease correction in adult stem cells isolated from two patients with cystic fibrosis (Schwank...

  7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...techniques may hold the key. In the simplest form, translating gene therapies from conceptual design to clinical... thought to be as high as 70% among the human population. These pre-existing antibodies can interfere ...

  8. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...the membrane for storage, but plan to reprobe it later, strip it thoroughly first. Then continue on with...always be added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use...

  9. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ... choose primary antibodies from more distantly related species and/or use narrowly specific secondary ... antibodies raised in different, and distantly related, species, and secondary antibodies raised in the...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...signaling in primary cells. The authors used this platform to assay dozens of GPCRs across many cell types...context-dependent GPCR activity with a universal biosensor platform. Cell, 187(6), 1527–1546.e25. doi: https://doi.org...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    .... Time Viral production in mammalian cells is relatively quick. After transfection, HEK-293T cells start... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...

  12. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...and Maria have lots of ideas and are happy to speculate, they know they can’t answer these questions alone...have collected a variety of data that might help formulate some hypotheses. They are excited to see other...

  13. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...’s best to turn to an established social media platform. During the conference If the conference has a...looking for a job - those connections can help you later on. Update your LinkedIn and your resume and prepare...

  14. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...recombination produces a minor pre-recombined population that does not require the recombinase for transgene... Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated...

  15. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...universities!) This guide tells you how to use online platforms, such as Zoom, to determine if a program is the... yours does and then use that time! Different platforms have different quirks, so remember to practice...

  16. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...easiest switch from fluorescent proteins given the relative simplicity of the system and the availability ...PMC2802578. 4. Xue, Lin, et al. "Imaging and manipulating proteins in live cells through covalent labeling...

  17. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...     The requirement for Cas9 transcription and translation delays editing What are Cas9-gRNA ribonucleoproteins... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...

  18. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...the hard work for you by immunizing an alpaca, isolating HCab antibodies from its serum, and meticulously...rapid epitope-mapping and native protein complex isolation. eLife. PubMed PMID: 26633879 PubMed Central PMCID...

  19. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...communities and into species not previously cultured or isolated (Rubin et al., 2020).  Figure 3: Multiplexed...-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR...

  20. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...expression, shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not...applications where a distinct subset of the cellular population needs to be targeted. Q6: Are transfer plasmids...
Showing: 641 - 660 of 795 results