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  3. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector...

  4. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...domain proteins, they can be expressed in bacteria, making nanobodies a recombinant and renewable anti-IgG...

  8. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...

  10. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...

  11. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary classification...

  12. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...functional before investing time and resources into making a stable cell line. When expressing a viral construct...

  13. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...//doi.org/10.1111/1574-6968.12241  Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved...

  14. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ... than engineering an entirely new protein. This makes it much cheaper to use Cas9 activators. This larger...

  15. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...gRNA targets in that region. The CRISPR Software Matchmaker can be used to determine the pros and cons of...

  16. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... generally ensure cell line identity and purity making it easier for comparable studies to be performed...

  18. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...gene! STEP 2: Generate an Expression Clone When making the expression clone, it is important to choose...
Showing: 641 - 660 of 698 results