We narrowed to 709 results for: MAK
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TypeBlog PostPublishedJune 7, 2018, 1:17 p.m....translated at the ribosome into polypeptide chains that make up the primary structure of proteins. Most proteins...
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xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....if you’d like to use homology directed repair to make precise edits or knock-ins. Editing efficiency decreases... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m.... protein characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based system for... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog PostPublishedAug. 29, 2019, 12:50 p.m....interested in viewing which has drawn a large crowd, making it difficult to see the actual poster or interact... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....target cells (Beatty et al., 2012). These features make AdV an attractive candidate for in vivo delivery... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....ability to differentiate into multiple cell types makes them useful models for developmental processes and... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....is required for NgAgo optimization before we can make any major conclusions: That said, many people are... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....domain proteins, they can be expressed in bacteria, making nanobodies a recombinant and renewable anti-IgG... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....process – such as the viral DNA polymerase – it makes sense that vectorization strategies include creating... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....synaptic transmission, are right around 250 nm, making it difficult to study the inner workings of the... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m.... Using this technique, researchers have begun to make important discoveries in various biological fields... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....added log-normal noise to each of the terms which make up our distribution, resulting in the following ... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....number of administrative and practical decisions to make. How often will you hold it? How many people do ... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....ve built a new and improved search engine to help make this task a faster, easier, and frustration-free... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....decide which ones to use? Here are some tips for making those decisions: Always always always look at ... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m.... DNA compaction and the structural proteins that make it possible, see the appendix DNA is a 3D fractal...