We narrowed to 708 results for: MAK;
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TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....that they don’t always require needles. If needles make you cringe or your experiment will allow you to ...
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Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....of rAAV production. Of course, you will want to make sure that your final stocks only contain rAAV. To... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....will ‘look’ similar on your flow plot. This will make it difficult to properly gate your desired cell ... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....degron will behave with a new protein, but you can make an informed choice! References and Resources References... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....if you’d like to use homology directed repair to make precise edits or knock-ins. Editing efficiency decreases... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m.... protein characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based system for... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....is required for NgAgo optimization before we can make any major conclusions: That said, many people are... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....added log-normal noise to each of the terms which make up our distribution, resulting in the following ... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....synaptic transmission, are right around 250 nm, making it difficult to study the inner workings of the... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....decide which ones to use? Here are some tips for making those decisions: Always always always look at ... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m.... Using this technique, researchers have begun to make important discoveries in various biological fields... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....number of administrative and practical decisions to make. How often will you hold it? How many people do ... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....ve built a new and improved search engine to help make this task a faster, easier, and frustration-free... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....process – such as the viral DNA polymerase – it makes sense that vectorization strategies include creating... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....domain proteins, they can be expressed in bacteria, making nanobodies a recombinant and renewable anti-IgG... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m.... DNA compaction and the structural proteins that make it possible, see the appendix DNA is a 3D fractal... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary classification...