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...inoculated from a colony on a plate will reach an optimal density in 18-24 hours. I then add 0.5 mL of this...

...The Root and Doench lab selected sgRNAs in this optimal window and ranked them based on their optimized...

...Select the model system for your research (e.g. Mammalian) Vector Type: What type of experiment are you ...

...benefits of lentiviral vectors have been observed in small trials for other diseases, including the common ...

... 2D barcode can store a lot of information in a small space and is ideal for tracking large collections...

...strategy builds on the steps listed above and formalizes your development. You may consider earning a ...

...serious relationship. #5 Thou shalt not sweat the small stuff The house can be messy and things won’t always...

...transcriptase (RT) variants, PE6a and PE6b are 500–800 bp smaller than previous editors, but just as effective and...

...demonstrating it. Requests like this are both very normal and very helpful; don’t be afraid to ask! Especially...

...website, we’ve learned about the importance of smaller, systemic changes, like using alt-text, captions...

... preparation for updating this post, I took an informal poll of Addgene hiring managers. All of them stated...

...titrating each lot of AAV in vivo to determine the optimal dosage. At Addgene, we don’t reserve lots, but ...

...also the proposed salary range) should help. In smaller companies, technical support is often covered by...

...PhDs found that many researchers feel they lack formal training in a variety of transferable skills. At...

...sensitivity, the DNA is first amplified through isothermal amplification by RPA. When Cas12a-cRNA base pairs...

...work environments outside of academia provide a formal training regime and the right set of tools to help...

...-Kulka, and Gad Glaser. "An Escherichia coli chromosomal" addiction module" regulated by guanosine [corrected...

...complex challenges, quickly breaking them up into smaller achievable tasks, and then executing on them in...

...Schematic representation of integration process and episomal forms of non-integrating transgenes. Created with...

...Substantive conflict occurs when people or teams who normally get along well with each other disagree about ...