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Showing: 641 - 660 of 840 results

  1. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...is placed downstream of the rest of the SPARC components and expression is controlled by the upstream ...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for...

  3. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...powerhouse depositors who have generously shared their reagents freely with the research community. Sharing is...logistics and facilitate all material transfer agreements, thereby freeing up time for our depositors to...

  5. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    .... To help you find the vector that fits your experiments, I've described below some of the most frequently...backbone. Or just ask –  leave a message in the comments below and an Addgenie will help you find what ...

  6. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...double nickase for my CRISPR genome engineering experiments? A1: When assessing which nickase type to use...use for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized chimeric...

  7. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...scientist. It is shocking how little pre-work most PhD students and postdocs do to ensure the advisors they choose... than once to get a full picture and listen for hints at the same concerns from multiple people. Listen...

  8. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...bacteriophages did not come to light until 2007. Initial experiments exposed S. thermophilus to predatory phages to... Cas proteins snip the foreign DNA into small fragments approximately 30bp in length and paste them into...

  9. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...samples, were engineered and adapted for RNAi experiments for mammalian cell lines. The Turner Lab Human...

  10. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...nucleic acid will be flanked by viral packaging elements that will ensure the virus gets produced, and ...What is a Virus? Viral Vectors 101: Viral Vector Elements ...

  11. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...transduction patterns empirically for their own experiments. To this end, we offer specific control AAV in...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...

  12. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ... with different CellTag libraries at multiple timepoints during reprogramming. These library-specific ... with different CellTag libraries at three time points during the 28 day study: prior to reprogramming...

  13. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...jGCaMP7 (keep reading) and will be releasing more variants in the next few months (see “coming soon”). The... can also be used as the control vectors for experiments encoding a fluorescent-tagged molecular tool....

  14. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...lysine residue within the AviTag. However, large amounts of the target protein are typically required due... site could be used for plasmid version 2.0? Instruments such as an Octet may require less in vivo-biotinylated...

  15. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...communicators also use the hero’s journey, stories from patients or other affected people, man/animal vs nature... the Addgene blog SSCS: Cognitive Load SSCS: Preprints Intro to our Summer SciComm Series  ...

  16. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory ON/OFF master switches...knowledge base to describe aptamers and SELEX experiments. Database, bas006 (2012). PubMed. For a stepwise...

  18. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...type of bacteria it is being used in, and how components of the plasmid should change based on bacteria...leading to safer medicine and more successful experiments. It’s something that all scientists should understand...

  19. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...feed. Due to increasing prices of methanol and advancements in other biomass production techniques in the...suited to it. Putting K. phaffii to work   The yeast giants - S. cerevisiae and S. pombe - aren’t going anywhere...
Showing: 641 - 660 of 840 results