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We narrowed to 849 results for: RING

Showing: 641 - 660 of 849 results
  1. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up as to whether it’s faster...cloning method Find blog posts about scientific sharing here Download the Plasmids 101 eBook Resources...
  2. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...spectral properties of the fluorophores you are considering to make sure that they’ll be compatible with ...target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores. We also touched on fluorophores...
  3. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...enough that there is virtually no chance of it occurring randomly. Therefore, inserting loxP sites at deliberate...are active only in certain cell or tissue types, during certain stages of development, or by making the...
  4. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...specific locations and give rise to progenitors during growth and development or that blood flows through.... While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these sequences...
  5. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...hold great promise for improving or potentially curing human diseases that were previously thought to ...as gene delivery tools: Unlike most naturally occurring viruses used in clinical trials, wild type AAV...
  6. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability ...Nanoblades editing efficiencies of primary cells. Comparing Nanoblades to Existing CRISPR Delivery Methods...
  7. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...spread. They can be inherited by daughter cells during cellular reproduction, similar to the host’s chromosome...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria to form...
  8. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...options are available to you, it may be worth considering an antibody raised in a different species.    ... strategically plan primary/secondary antibody pairing such that each target has a unique species or isotype...
  9. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ... of Research (FoR) symposia held across the USA during this time have revealed that young scientists are...Biochemistry and Molecular Biology/ FoR Hack Day during which several projects were initiated. Recent developments...
  10. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...and Ghyselinck NB. A directional strategy for monitoring Cre-mediated recombination at the cellular level...Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector...
  11. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...change (facilitated by the flexible linker) that brings the fluorescent units of the FRET pair together... and diabetes mortality determined by nutrition during parents' and grandparents' slow growth period."...
  12. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...their mental model of the protein. At some point during this interrogation process, they will almost invariably...recognize a PAM sequence, allow the complementary base pairing between the guide RNA and the target DNA strand...
  13. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR tagging...
  14. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...the Challenges of Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...
  15. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...expression baseline. Copy number is then determined by comparing results to a standard curve. For CNV assays, it...gives an absolute value of DNA concentration. By comparing the number of positive droplets for the gene of...
Showing: 641 - 660 of 849 results