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We narrowed to 855 results for: RING

Showing: 641 - 660 of 855 results
  1. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific symposium...inspiration to resolve the technical hurtle of monitoring protein turnover of specific proteins in situ...
  2. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...spectral properties of the fluorophores you are considering to make sure that they’ll be compatible with ...target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores. We also touched on fluorophores...
  3. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...enough that there is virtually no chance of it occurring randomly. Therefore, inserting loxP sites at deliberate...are active only in certain cell or tissue types, during certain stages of development, or by making the...
  4. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether... and the concentration of the protein solution. During the experiment, the solution is diluted, complicating...
  5. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ... could present something coherent or to quickly string together all the words that I could remember related...conferences, or even just provide quick updates during a company meeting increases visibility of your ...
  6. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up as to whether it’s faster...cloning method Find blog posts about scientific sharing here Download the Plasmids 101 eBook Resources...
  7. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...similar to a Fab fragment but created through engineering instead of cleavage. They are comprised of the...stable up to 80°C and can refold correctly after denaturing.  Designed ankyrin repeat proteins, or DARPins...
  8. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...informing potential for success in a patient-mutation bearing knock-in mouse model for CTD. Congratulations the...questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building upon...
  9. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability ...Nanoblades editing efficiencies of primary cells. Comparing Nanoblades to Existing CRISPR Delivery Methods...
  10. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...specific locations and give rise to progenitors during growth and development or that blood flows through.... While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these sequences...
  11. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...change (facilitated by the flexible linker) that brings the fluorescent units of the FRET pair together... and diabetes mortality determined by nutrition during parents' and grandparents' slow growth period."...
  12. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...and Ghyselinck NB. A directional strategy for monitoring Cre-mediated recombination at the cellular level...Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector...
  13. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...spread. They can be inherited by daughter cells during cellular reproduction, similar to the host’s chromosome...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria to form...
  14. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ... of Research (FoR) symposia held across the USA during this time have revealed that young scientists are...Biochemistry and Molecular Biology/ FoR Hack Day during which several projects were initiated. Recent developments...
  15. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...hold great promise for improving or potentially curing human diseases that were previously thought to ...as gene delivery tools: Unlike most naturally occurring viruses used in clinical trials, wild type AAV...
  16. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...options are available to you, it may be worth considering an antibody raised in a different species.    ... strategically plan primary/secondary antibody pairing such that each target has a unique species or isotype...
  17. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...the Challenges of Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...
Showing: 641 - 660 of 855 results