We narrowed to 877 results for: TOR

...neurons that trigger the CPG (central pattern generator) (Wyart et al., 2009). The CPG is a neural circuit... involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...optogenetics blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org...

... gene in a pLenti vector backbone. During those five weeks, I worked with my mentors, Katherine, Amrita...exchanged contact with Amy C., Addgene’s intern coordinator shortly after hearing her talk about Addgene’...

...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...array: The section of the bacterial chromosome that stores a genetic record of previously encountered foreign...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

...stuffing new depositor welcome kits, note taking for protocol video production, and keeping inventory of our... the people at Addgene have become my friends, mentors, and some of my favorite people to talk to. This...

...original policy was straightforward, but cumbersome: depositors had to email us with permission to release their... are left in the hold status and reach out to depositors about them, but we all know how easy it is to...your work. Plus, it’s costly for us to process and store materials that aren’t distributed or for our teams...

...affiliated TniQ protein recruits transposition cofactors. The transposon proteins integrate a payload sequence... Figure 1: Mechanism of CAST systems. A Cas effector (Cascade in Type I systems; Cas12k in Type V-K...Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase. Science...

...match sequence information contributed by the depositor, published backbone sequences, and any known sequences...analyze sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps...experiments like restriction digests. We’ll have a tutorial showing all of the features available on our Snapgene...

...patient partners so that they can help tell their stories. I was paired with a six-year-old boy named AJ ...caused by a single R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate bone regeneration...where my wife Lianna has made her career as Senior Director of Biology, is helping us fight against FOP in...

...you questions. Remember that people like a good story and science involves characters – in this case you...or other places online, and consider how those stories are being told. Twitter can be a good way to follow...sharing – both your plasmids and your research stories. Consider practicing communicating your research...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...2018 PubMed PMID:29109157. Nanobody toolkit to monitor retrograde transport at the cell surface Article...able to bind fluorescent proteins derived from A. victoria the functionalized nanobodies described here can...

...meaningful day job only came into focus when an English tutor noted my eclectic mix of science and arts papers...venturing forth from tertiary study, the myriad trajectories ahead can be condensed into two discernible ...truly benefit others. Herein, a good science communicator—with an understanding of how to appeal to a ...

...research materials, such as plasmids and viral vectors. However, it typically takes a few years for a .... Daniela Bourges, Addgene’s interim Executive Director. “NABOR provides a new model for the creation ... the NIH’s BRAIN Initiative has been funding laboratories, such as Dr. Trimmer’s lab, to create recombinant...

...will describe how to copy a cDNA from one vector into a new vector that is better suited for analyzing the...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...

.... I had just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge and knew...outside of academia, a job as a ‘Cancer Marketing Coordinator’ came up at Abcam. Unlike other job descriptions...knew there would be a way around this. First off I tore up my “Scientific CV” and wrote a more business ...

...who may not be experts, can you give me a brief history of optogenetics? How long has this idea been around...optogenetic tools for neuroscience back in 2005, both activators as well as silencers of neural activity. Since...brain is very complex and we've hosted hundreds of visitors in our lab to help them understand their specific...

...interactions and growth within the biofilms will then be monitored using fluorescence microscopy and FACS. In an ...to create gene deletions of important unknown regulatory regions in Pseudomonas and Klebsiella spp. The...projects to reach audiences beyond our plasmid depositors and requesters. Check out some of our recent ...

...great deal more information that one might like to store about FPs than is currently represented at fpvis...a centralized, community-editable resource for storage and dissemination of information about FPs. FPbase.org...elaborate spectrum analysis tools, and a better way to store and represent within-study photobleaching comparisons...

... plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think we missed...expression of the tumor-seeking CAR (chimeric antigen receptor), whereas a ubiquitous promoter drives expression...can be made for expressing your favorite T-cell receptor, or perhaps any other tool that can benefit from...

...classification system, including its history, biology, and usages in the lab. History In 1884, while studying infected...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...

...correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination in cultured...control the CRISPR system activity.  Using an AAV vector may help with size limitations. In addition, non-viral...