We narrowed to 745 results for: TRI

1. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...not be accessible by wild-type Cas9, due to its stringent PAM specificity. This can be especially important...

2. Targeted Mutagenesis with EvolvR

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   Blog Post

   Published

   Feb. 21, 2019, 1:15 p.m.

   ...within a defined region of a genome. Require stringent bacterial growth conditions. Expression of an ...

3. A Quick Guide to a Career in Software Product Management

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   Blog Post

   Published

   Nov. 24, 2020, 2:15 p.m.

   ...This post was contributed by guest blogger, Rachel Rubinstein, a field-based software product manager...

4. Plasmids 101: Screens vs. Selections

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   Blog Post

   Published

   Nov. 10, 2022, 2:15 p.m.

   ...selection can take the form of varied temperature, nutrient availability, drugs, and much more.   Workflow...

5. Antibodies 101: Isotypes

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   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...edition. New York: Garland Science; 2001. The distribution and functions of immunoglobulin isotypes. Available...

6. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ..., so it no longer overlaps with the control. In tricky experiments, these populations may overlap slightly...

7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...structural protein of the retroviral particle containing Matrix, Capsid, and Nucleocapsid components. Pol ...

8. Viral Vectors 101: Biosensors

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   Blog Post

   Published

   July 6, 2023, 1 p.m.

   ...with dozens of sensors developed for it. Calcium triggers muscle contraction, and calcium channels are activated...

9. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...witnessed several colleagues do this while changing industries and positions. Some people discover they truly...

10. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    Published

    June 6, 2023, 1:15 p.m.

    ...efficiency. However, gamma-retrovirus infection is restricted to dividing cells only, so is not suitable for...

11. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    Published

    April 27, 2023, 1:15 p.m.

    ...about a hundred billion neurons and six hundred trillion connections (synapses.)  In this post we’ll describe...

12. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...multifragment assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis...

13. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...epitope. Addgene: Do you have any additional tips, tricks, or words of advice for people who may want to ...

14. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...of their CRISPR work in rice plants. Yang was intrigued by the tRNA processing machinery present in almost...

15. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...was a little like tapping in the dark - a lot of trial and error.” The structural details revealed two ...

16. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post

    Published

    May 22, 2014, 4:02 p.m.

    ...This post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes...

17. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ... with viral RNAs. RNA viruses, like Zika, often trick host proteins into binding their untranslated regions...

18. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...This post was contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize...

19. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    Published

    Dec. 8, 2022, 2:15 p.m.

    ...package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently...

20. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal...