We narrowed to 745 results for: TRI
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TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....not be accessible by wild-type Cas9, due to its stringent PAM specificity. This can be especially important...
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Targeted Mutagenesis with EvolvR
TypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m....within a defined region of a genome. Require stringent bacterial growth conditions. Expression of an ... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....This post was contributed by guest blogger, Rachel Rubinstein, a field-based software product manager... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m....selection can take the form of varied temperature, nutrient availability, drugs, and much more. Workflow... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....edition. New York: Garland Science; 2001. The distribution and functions of immunoglobulin isotypes. Available... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m...., so it no longer overlaps with the control. In tricky experiments, these populations may overlap slightly... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m....structural protein of the retroviral particle containing Matrix, Capsid, and Nucleocapsid components. Pol ... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....with dozens of sensors developed for it. Calcium triggers muscle contraction, and calcium channels are activated... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog PostPublishedJan. 21, 2025, 2:15 p.m....witnessed several colleagues do this while changing industries and positions. Some people discover they truly... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....efficiency. However, gamma-retrovirus infection is restricted to dividing cells only, so is not suitable for... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....about a hundred billion neurons and six hundred trillion connections (synapses.) In this post we’ll describe... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....multifragment assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....epitope. Addgene: Do you have any additional tips, tricks, or words of advice for people who may want to ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....of their CRISPR work in rice plants. Yang was intrigued by the tRNA processing machinery present in almost... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....was a little like tapping in the dark - a lot of trial and error.” The structural details revealed two ... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog PostPublishedMay 22, 2014, 4:02 p.m....This post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research. With many changes... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m.... with viral RNAs. RNA viruses, like Zika, often trick host proteins into binding their untranslated regions... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....This post was contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal...