We narrowed to 738 results for: TRI
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TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....edition. New York: Garland Science; 2001. The distribution and functions of immunoglobulin isotypes. Available...
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Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog PostPublishedMay 3, 2018, 1:48 p.m....activity. This property makes WYL proteins an intriguing counterpart to anti-CRISPR proteins that negatively... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....translation. Many thanks to Nicole Zurcher who contributed to the writing of this article. Additional Resources... -
Targeted Mutagenesis with EvolvR
TypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m....within a defined region of a genome. Require stringent bacterial growth conditions. Expression of an ... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....not be accessible by wild-type Cas9, due to its stringent PAM specificity. This can be especially important... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....This post was contributed by guest blogger, Rachel Rubinstein, a field-based software product manager... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....multifragment assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....epitope. Addgene: Do you have any additional tips, tricks, or words of advice for people who may want to ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....of their CRISPR work in rice plants. Yang was intrigued by the tRNA processing machinery present in almost... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....was a little like tapping in the dark - a lot of trial and error.” The structural details revealed two ... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog PostPublishedMay 22, 2014, 4:02 p.m....This post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research. With many changes... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....mechanisms. Most take advantage of chemical groups intrinsically present on antibodies, such as the primary amine... -
Viral Vectors 101: An Introduction to AAV
TypeBlog PostPublishedMay 30, 2023, 1:15 p.m....relevant viruses for your system. This post was contributed by guest blogger Didem Goz Ayturk with edits ... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m.... could present something coherent or to quickly string together all the words that I could remember related... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m.... to genome instability. Thus, it’s important to strike a balance between productive levels of R-loops ... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....This post was contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m.... with viral RNAs. RNA viruses, like Zika, often trick host proteins into binding their untranslated regions... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....at Novartis. The ClonTracer library has been distributed all over the world and was recently used in a...