We narrowed to 812 results for: cha

...soluble and insoluble proteins, which can be a challenge for classic antibody based affinity purification..., S. C., Noble, K. A., KC, B., Roux, K. H., Motamedchaboki, K., & Roux, K. J. (2016). An improved smaller...

...might suggest, an RNA-mediated gene silencing mechanism. RNAi was a landmark discovery in the 1990s and..., C. (2018). Overview of MicroRNA biogenesis, mechanisms of actions, and circulation. Frontiers in Endocrinology...

...enzyme screening is sensitive to single base pair changes, but the edit must occur at an existing restriction...simultaneously validate edits and look for off-target changes. This method requires a set of control cells to...

...role of renaturation temperature in the polymerase chain reaction." Analytical Biochemistry 209.2 (1993):...Xia, Yongzhen, et al. "New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase...

... a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence specificity.... Ramanan V, Shlomai A, Cox DB, Schwartz RE, Michailidis E, Bhatta A, Scott DA, Zhang F, Rice CM, Bhatia...

...competent cells. The master mix of enzymes can be purchased from a company (e.g. NEB or SGI-DNA), or can be...fragments of the chromosome, which was technically challenging. Other homology based technologies We've described...

...below outlines a few of the more common genetic changes found in E. coli strains. Table 1: Common gene ...are methylation sensitive. dnaJ Mutation in a chaperonin gene Increases the stability of certain expressed...

... using proper technique, there’s always a small chance that a researcher could infect him or herself with...you put in your culture. As viral production can change from one batch to another. For experimental consistency...

... genomic DNA to amplify the gRNA targets was challenging because you’re limited by the sequence of the...Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol...

...Watson, Crick, & Franklin, Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make...process that kits require; Nicole, the web master in charge of all our externally facing kit pages. No single...

...the C’ terminus of the protein, to maximize the chances of creating a non-functional allele. For a 1 kilobase...the so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees et al., 2018...

...were inspired by the imminent threat of climate change to study plant and marine biology, environmental...and while doing scientific research. It’s okay to change your mind about what exactly you want to do—I started...

...Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited...is equally important to have a plan to get to mechanism. Do the necessary tools exist to tackle the problem...

... editing or labeling efficiency doesn’t usually change when you add multiple gRNAs on one plasmid. Sound...contains all of the desired gRNAs. This increases the chance that all the edits you want to make will happen...

... is working for you, then there is no reason to change it. But what if a kit isn’t working for you, or...factor label Non-silica based, avoids use of chaotropic salts   What are all these other kit types...

...indicate the amino acid change as well as any functional consequences of that change. For example, "Mutated...

...mammals.      Subscribe to Addgene's YouTube channel for more helpful animations, useful protocol videos...

...Haery has summed up some of the most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses...

...our TikTok! Be sure to subscribe to our YouTube channel for quick tips, protocols, animations, and more...

...you can watch the full animation on our YouTube channel.  More resources on the Addgene blog Antibodies...