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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 852 results for: ell

Showing: 641 - 660 of 852 results

  1. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ...you feel about dissecting a cat?' And I’m like, 'Well, I’m here so let’s do it!'" Working as a subject...inside. We do a lot of interactives and videos, as well, to supplement the hands-on [aspect of the labs]...interviewed, ‘I write science. I edit science. And I do it well.’” Stay tuned for the next feature in this series...

  2. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...precise mechanistic details of the process are not well understood, it is believed that the exonuclease ...properly. Directly transform chemically competent cells with the digested mixture. There is no need for ...purification before transformation. After transforming cells, you should perform colony PCR with vector specific...

  3. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...paired with patient partners so that they can help tell their stories. I was paired with a six-year-old ...similar age. When he came to visit us and watch fellow Run4Rare team members complete the Boston Marathon... scientific community know, treatments that work well in animal models do not always work as efficiently...

  4. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ... a range of different scientific disciplines, as well as social and networking events. Imperial Synthetic... networking opportunities for society members as well as arranging collaborations and conferences for ...believe it’s very important to develop a network of fellow scientists that you can grow with, learn from, ...

  5. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...'t all career skills leaderhsip skills when done well? Do you just have to know it when you see it? “The...Managers must ensure that their teams are working well together and collaborating effectively with other...the doing. Many science managers or leaders will tell you how much they miss the hands-on bench work. ...

  6. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...will have opportunities to work on Emotional Intelligence or to hone delegation skills, for example. So...their managers. You can’t just think it, you have to tell them. A good manager learns that her success and...dissatisfaction, the work can get done, and get done well. The rest of the blogs in this series will address...

  7. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...the world.  Nina Dudnik, Founder of Seeding Labs, tells the story about working as a student in a rice lab...I was in graduate school in the 80’s, my lab was well funded and we didn’t feel the need to reuse plasticware...recently shared our protocol for making competent cells to save time and money. My other stingy science ...

  8. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...Consider reading Toxic Academic Mentors by @drmellivora, an excellent blog post about workplace bullying featured...the first place. The good news There are many excellent mentors out there. With some good pre-work you...

  9. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...fully open and available to industry requestors as well. This is a time when we need as much open science...accelerates and enhances productive collaborations as well as provides visibility for grant-driven research...scientists worldwide with Addgene’s traditional excellence through whatever lies ahead this year. We can...

  10. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...the number of added biotins per molecule cannot be well-controlled. The lack of site selectivity typically...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently... Eye Institute and is particularly interested in cell signaling.     Additional Resources on the Addgene...

  11. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...Academic Solos to Industrial Symphonies, an article well worth reading on this topic, the authors state, ...colleagues and friends why he would give up,“an excellent—some say “cushy”—tenured faculty position for ...especially good at self-promoting—No one is going to tell you to pick up the pace Constant worry about grants...

  12. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    Published
    Nov. 8, 2018, 3:47 p.m.
    ...scientist at MIT’s Computer Science and Artificial Intelligence Lab and a Visiting Professor at the Lab for ...Classifying genes as natural or synthetic But how can you tell if a gene is synthetic or natural? The answer to...distantly related organism, the gene may not express well because of these different codon usage biases. But...

  13. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ... road, science communication graduates are often well-equipped. Many institutions are sincerely interested...carried over and allowed to percolate within a new intellectual environment. Over time, this interest came to...risk-taking behavior among collision sport athletes, as well as the ethical and health policy implications arising...

  14. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...unhealthy, difficult-to-inject unc-119(ed3) animals, as well as a second injection step to remove the selection... promoter, creating a loss-of-function allele as well as a promoter-FP fusion for the gene of interest...systems may prove effective in other species or in cell culture. In addition to CRISPR/Cas9 applications...

  15. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...academic institutions.  What is the UBMTA, you ask? Well, organizations used to negotiate individual MTAs...materials are widely varied with many properties, having well-understood standardized terms makes it a lot easier...MTA most plasmids at Addgene are shared under, is well-understood and accepted by the majority of research...

  16. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...is something that absorbance based methods can’t tell you and you don’t need a spectrophotometer or a ...concentrations. Many commercial DNA ladders will tell you the concentration of each band in the ladder...Unlike absorbance based methods, this method doesn’t tell you about contaminating proteins or chaotropic salts...

  17. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...modern biological research.  Any microscopist will tell you that selection of a fluorescent probe (be it...  There are many online guides – including an excellent series of posts by Joachim Goedhart on the Addgene...Associate at Harvard Medical School. He manages the Cell Biology Microscopy Facility, specializing in advanced...

  18. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...processes in the Addgene lab. In this post, I’ll tell you a little bit about what I learned from a New...newly created libraries were analysed to see how well the kit worked. As NEB hoped, we found that regardless...day was Julie from Novartis who was working with cell culture and who was extremely helpful since she ...

  20. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...both the approximate size of the vector backbone as well as the predicted size of the insert. You can search...buffer will make sure your sample settles in the gel well and the dyes provide a visual reference point so...Bonus: knowing how much DNA you have loaded in each well will allow you to approximate the DNA mass of comparably...
Showing: 641 - 660 of 852 results