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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 845 results for: ins;

Showing: 641 - 660 of 845 results

  1. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...targets, you might decide to do simultaneous IF instead (Figure 1B). To label all your targets in one step...yourself. If you can’t find a suitable antibody against your target protein, consider fusing a protein ...Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex Immunofluorescence on Fresh...

  2. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ... a summer role as a Research Scientist at the Institute of Cancer Research in Sutton, located on the edge...and plenty of jobs in science at world renowned institutes. The lab at the University of Queensland required...field don’t really get to do a lot of lab work but instead really need to be comfortable with programming....

  4. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...used GAPDH has noted issues as a reference gene (Higgins, 2000; Ref, Zhu, 2002), though there are cases ...run and the difference in CT values is plotted against the log input concentration for each dilution. ...://doi.org/10.1038/s41598-018-26707-8Suzuki T; Higgins PJ; Crawford DR. Control selection for RNA quantitation...

  5. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...a template. To do so, they used a Cas nickase, instead of dCas9. The resulting editor, BE3, nicks the ...and/or evolved to catalyze cytidine deamination instead of (or in addition to) adenosine deamination (Chen... R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained genome targeting...

  6. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...Bluesky: @addgene.bsky.social; or @addgene on Instagram, TikTok, Facebook, and LinkedIn. We are also exploring...and features that previously made the platform a mainstay of conversation in the scientific community. Addgene...

  7. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ...of our holiday decorations, our holiday spirit remains. We would like to thank everyone who participated...creating memories with their labmates! Are you inspired by our 2017 #Deckthelab winners? Be sure to enter...

  10. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...CMV promoter. Envelope proteins are a key component of lentiviruses. These proteins coat the virus and help...

  11. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...The Baker Lab focuses on designing entirely new proteins, improving our understanding of how protein folding...and others. The introduction of de novo designed proteins also helped researchers understand more about ...

  12. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...Goldilocks Antibody: Guest blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies... a drag-and-drop CRISPR editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest ...

  13. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...Plasmid Kits for microbial work with fluorescent proteins, synthetic biology, gene cloning and expression...them on our search page. Use the Expression and Insert Species filters on the right-hand side of the search...

  15. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...to cut other DNA or RNA sequences. Nucleases: Proteins that bind to gRNAs and are directed by the gRNAs...prime editing and the identification of other Cas proteins continue to expand the targeting range. CRISPR...

  16. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...tradition in which we choose our teams and start brainstorming months in advance. Our teams comb the news (...Friday. So if you’re interested in finding out who wins this year’s Addgene costume contest, like us on ...

  17. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...and metabolism. Melina has worked at Genetics Institute in Cambridge, MA and Sequana Therapeutics in San...the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control Addgene's...

  18. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ... share their AAV validation data. The Data Hub contains a curated collection of community submissions ...) can save you significant time and money. For instance, this report shows that anterograde, monosynaptic...

  19. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...academic institutions can afford to hire. It will be difficult for some of these institutions to manage...

  20. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ... about how fluorophores other than fluorescent proteins can be incredibly useful in FRET experiments. ... our posts if you have any questions or useful insights. If you're interested in blogging for us, fill...
Showing: 641 - 660 of 845 results