Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 793 results for: lat

Showing: 641 - 660 of 793 results

  2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...techniques may hold the key. In the simplest form, translating gene therapies from conceptual design to clinical... thought to be as high as 70% among the human population. These pre-existing antibodies can interfere ...

  4. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...correcting monogenetic diseases. Now, almost 10 years later, CRISPR’s potential for treatment is no longer an...CRISPR to disease correction in adult stem cells isolated from two patients with cystic fibrosis (Schwank...

  5. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ... choose primary antibodies from more distantly related species and/or use narrowly specific secondary ... antibodies raised in different, and distantly related, species, and secondary antibodies raised in the...

  6. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system takes the...Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable marker; attB site...

  7. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...the membrane for storage, but plan to reprobe it later, strip it thoroughly first. Then continue on with...always be added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use...

  8. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...recombination produces a minor pre-recombined population that does not require the recombinase for transgene... Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated...

  9. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...’s best to turn to an established social media platform. During the conference If the conference has a...looking for a job - those connections can help you later on. Update your LinkedIn and your resume and prepare...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...signaling in primary cells. The authors used this platform to assay dozens of GPCRs across many cell types...context-dependent GPCR activity with a universal biosensor platform. Cell, 187(6), 1527–1546.e25. doi: https://doi.org...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    .... Time Viral production in mammalian cells is relatively quick. After transfection, HEK-293T cells start... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...

  12. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...and Maria have lots of ideas and are happy to speculate, they know they can’t answer these questions alone...have collected a variety of data that might help formulate some hypotheses. They are excited to see other...

  13. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...universities!) This guide tells you how to use online platforms, such as Zoom, to determine if a program is the... yours does and then use that time! Different platforms have different quirks, so remember to practice...

  14. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...the hard work for you by immunizing an alpaca, isolating HCab antibodies from its serum, and meticulously...rapid epitope-mapping and native protein complex isolation. eLife. PubMed PMID: 26633879 PubMed Central PMCID...

  15. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...     The requirement for Cas9 transcription and translation delays editing What are Cas9-gRNA ribonucleoproteins... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...

  16. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...communities and into species not previously cultured or isolated (Rubin et al., 2020).  Figure 3: Multiplexed...-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR...

  17. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...easiest switch from fluorescent proteins given the relative simplicity of the system and the availability ...PMC2802578. 4. Xue, Lin, et al. "Imaging and manipulating proteins in live cells through covalent labeling...

  18. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...expression, shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not...applications where a distinct subset of the cellular population needs to be targeted. Q6: Are transfer plasmids...

  19. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...session is to start with something completely unrelated to the issue and consider how it could be shaped...problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for...

  20. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...; the importance of which will be elaborated on later. As for what job fairs to consider, some of the ...the way. Make connections, and nurture those relationships. Then when you are ready to apply for your dream...
Showing: 641 - 660 of 793 results