We narrowed to 713 results for: mak

1. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ...ability to differentiate into multiple cell types makes them useful models for developmental processes and...

2. Plasmids 101: Protein Expression

   Type

   Blog Post

   Published

   June 7, 2018, 1:17 p.m.

   ...translated at the ribosome into polypeptide chains that make up the primary structure of proteins. Most proteins...

3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ...target cells (Beatty et al., 2012). These features make AdV an attractive candidate for in vivo delivery...

4. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   Published

   July 16, 2024, 1:15 p.m.

   ...of rAAV production.  Of course, you will want to make sure that your final stocks only contain rAAV. To...

5. Antibodies 101: Flow Compensation

   Type

   Blog Post

   Published

   May 16, 2024, 1:15 p.m.

   ...will ‘look’ similar on your flow plot. This will make it difficult to properly gate your desired cell ...

6. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ... protein characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based system for...

7. Hot Plasmids: Winter 2025

   Type

   Blog Post

   Published

   March 11, 2025, 1:15 p.m.

   ...and more photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques...

8. Common Injection Routes in Mice

   Type

   Blog Post

   Published

   Jan. 22, 2025, 9:42 p.m.

   ...that they don’t always require needles. If needles make you cringe or your experiment will allow you to ...

9. Plasmids 101: Degron Tags

   Type

   Blog Post

   Published

   March 14, 2024, 1:15 p.m.

   ...degron will behave with a new protein, but you can make an informed choice!   References and Resources ...

10. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...if you’d like to use homology directed repair to make precise edits or knock-ins. Editing efficiency decreases...

11. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

12. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    Published

    Aug. 4, 2016, 2:30 p.m.

    ...is required for NgAgo optimization before we can make any major conclusions: That said, many people are...

13. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    Published

    Dec. 3, 2019, 11:38 a.m.

    ...ve built a new and improved search engine to help make this task a faster, easier, and frustration-free...

14. Isolating Genomic Regions of Interest with the CRISPR System

    Type

    Blog Post

    Published

    May 30, 2019, 12:44 p.m.

    ... Using this technique, researchers have begun to make important discoveries in various biological fields...

15. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...

16. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    ...started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector...

17. CRISPR 101: RNA Editing with Cas13

    Type

    Blog Post

    Published

    July 31, 2020, 12:30 p.m.

    ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...

18. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ...added log-normal noise to each of the terms which make up our distribution, resulting in the following ...

19. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    Published

    Aug. 30, 2022, 1:15 p.m.

    ...decide which ones to use? Here are some tips for making those decisions: Always always always look at ...

20. Getting to Know Expansion Microscopy

    Type

    Blog Post

    Published

    June 20, 2023, 1:15 p.m.

    ...synaptic transmission, are right around 250 nm, making it difficult to study the inner workings of the...