We narrowed to 629 results for: Dos;

...detection range of your protein. The most common way to do this is via a Bradford assay or BCA assay. Be sure...preserve specific protein modifications, we recommend doing a little more research into gel chemistry and/or...wide dynamic range for fluorescent proteins. They do tend to have increased background with higher exposure...your blot to calculate the amount in your sample. (Do ensure your blot is optimized so your standard curve...

...our favorite posts from 2022! Wow! Our depositors do some really incredible science - I hope you've enjoyed...

...fluorescence when excited with ultraviolet (UV) light, they do so via aromatic amino acids found in all proteins...photons excite electrons of a fluorophore, they can do so at a range of wavelengths, rather than at just...1, emission spectra of neighboring colors can and do overlap. For example, APC overlaps noticeably with...

...biochemistry researcher at New England Biolabs. When do you need a high fidelity ligase—and when is an alternative...base pairing through minor grove contacts, and thus do not read specific base sequences, but are sensitive...differential is not known for certain, but may have to do with the slight melting of the 5 ́-terminus during...

... a part of a community that recognizes doing science (and doing it ethically) runs hand in hand with policy...views represented below are those of the writers and do not necessarily represent the views of Addgene. ...

... = 500,000 colonies.  Pro tip! Since you probably do not want to individually count 500,000 colonies, ...did not result in significant recombination. If you do see recombination, we recommend ensuring the cells...

...use But exactly what kind of editing are people doing with all these CRISPR plasmids (and libraries)? ...scientists use CRISPR technology? We think so — and so do our requestors! CRISPR plasmids in the Addgene repository...

...and realize you need to apply different filters you do not need to start a new search. Simply click the ...purpose (e.g. CRISPR) Beyond search What can you do if you don’t have any specific search terms in mind...

...newer, such as the relatively recent resurgence of “Do-It-Yourself Science” described in Chapter 3, and ...never inherently bad or evil. However, it is easy to do sloppy science, misrepresent science, or misuse science...

...Do you ever wonder about the origins of some of the common techniques or tools you use in the lab? Take... to be less sensitive to sequence context.    So, do Schüchner, et al.’s results mean the end of 9E10’...