We narrowed to 783 results for: INA

... nuclease domain. These mutations result in an inactive RNA-guided DNA binding protein (dCas9). dCas9 ...the next 3 ranked sgRNAs. Dolcetto was able to discriminate between non-essential and essential genes similarly... an improved activator design that consists of inactive Cas9-VP64, a sgRNA with two MS2 RNA aptamers, ... active sgRNAs for CRISPR-Cas9–mediated gene inactivation." Nature biotechnology 32.12 (2014): 1262. PubMed...

...proteins of interest has occurred. Although the original Y2H systems utilized the yeast Gal4 activator,...for screening greater numbers, but the latter eliminates the step of identifying prey proteins after screening... the experiment to reduce the likelihood of indiscriminate reporter gene activation. Including a prey-... libraries can be screened using both N- and C-terminal fusions of these proteins. This way both ‘ends...

... bit of Addgene history... As you may be aware, Melina Fan, her brother Ken Fan, and her husband Benjie... corn toss boards, Kan Jam, and Ladder Ball (I finally discovered my hidden talent in Ladder Ball... there...popularly- cookies). 3. Do something outside of your ordinary environment Team building events like Escape the...Veckerelli is the Administrative and Operations Coordinator at Addgene. In addition to her operational responsibilities...

...but some applications have been challenging or inaccessible. The Ting lab recently made two deposits that... Figure 3: A) LOV-Turbo becomes active when illuminated with blue light, catalyzing the biotinylation...that had been trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023.... (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...

... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...catalytically dead dCas13b to the catalytic domain of RNA deaminase ADAR2. Constructs containing the ADAR2 truncation...orthologs works for some applications, it doesn’t eliminate the need to deliver larger cargo such as base ...Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated...

...Microbe meeting, but it was my favorite because I finally felt like I had a network of people to talk to ..., Kat Milligan-Myhre, and Pragati Chengappa.  Financial and moral supporters Denise Akob, Sara Alexander...Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg, Heather Martinez, Pat Schloss, Tara Smith...McGovern, and Julie Wolf. Apologies to anyone I’ve inadvertently left off this list. Many thanks to our guest...

...suitable partner, connect the relevant teams, coordinate implementation, and now must focus on managing...some microscopy enthusiasts to start a microscopy seminar to connect the imaging community and to create ...university approachable, friendly, and helpful. Finally, don’t forget about collaborations and projects...for lunch, make a point of talking to them at a seminars, or even set up regular meetings. Meeting people...

...this is where most of our conference room names originated from. Let’s take a look at some of the stories...known to play a role in neural development, sex determination, and anterior-posterior segmentation (Jennings... SB transposon system including those from the original publication, Ivics et al 1997. Spaghetti Squash...may think that a gene called "spaghetti squash" originated from the squash itself, but think again. Spaghetti...

...their best interest to do so, sure, but it also eliminates that feeling of floundering one can have when...also need to talk to Tony from legal, Diana in finance, and Wu in graphic design. You could dread these...career options are expanding. As someone who coordinates cross-team projects at Addgene, I find that my... that led you into science in the first place. Finally, if you still find the idea of not holding a pipette...

... a second recombination event between the two flanking repeats. In cells where recombination has not occured... serves as a counterselectable marker in the destination vector. Successful cloning occurs when the ccdB...6308648. PubMed Central PMCID: PMC384129. Aizenman, Einat, Hanna Engelberg-Kulka, and Gad Glaser. "An Escherichia...