We narrowed to 884 results for: ica

...protein composition. It is used for a variety of applications such as measuring target protein expression ...expression levels, assessing post-translational modifications, determining cell health, analyzing cell cycle stages...of the population as a whole, scientists gain statistical power in their observations. However, while flow...in single-file where it is interrogated by the optical system (Figure 1).   Figure 1: Cells expressing...complexity, measured using visible light scatter The optical system has both visible and fluorescent light sources...cell expressing low levels of the protein. The electrical system converts the fluorescence intensity to...intensity events are assigned to higher channels. In a typical assay a user will compare the shifting intensity...

...study its function is one of the most popular applications of fluorescent proteins. These fusion proteins... from Aequorea victoria and its variants are genetically encoded fluorescent probes. One of the limitations...motifs that are necessary for post-translational modifications. Despite these potential issues, GFP has been...ultracentrifugation or gel filtration is the most common analytical method. However, these may not reflect the situation...situation in cells. Therefore, the localization of critical fusions (e.g. tubulin or connexin) has been used...linker sequence short In the early days of GFP applications, many were concerned with steric hindrance by...fluorescent protein and protein of interest (POI). To indicate the sequence, people often use terminology like...

...cryogenic electron microscopy (cryo-EM) protocols typically require high concentration and purity of the target... (Figure 1). This enrichment streamlines the purification and minimizes sample loss while also supporting...chaperone NPM2, but the technique should be widely applicable to other targets. The modular system of linkers...scFvs for other targets or could be used for applications beyond cryo-EM.   Find plasmids for MagIC-Cryo-EM...interact with the air-water interface (AWI) before vitrification, they can be completely denaturated (left) or...right). B) Protein samples protected from AWI by helical LEAs. C-E) cryo-EM single-particle analysis of ...orientations, this could be overcome with methods such as chemical crosslinking or tilted-stage imaging. A truncated...

...components of the cytoskeleton. They provide cells with physical structure, separate chromosomes during mitosis...filopodia formation, cell contraction, or other morphological changes, depending on the specific Rho GTPase...RhoGEF is a great way to stimulate and study these physical processes. Joachim Goedhart and colleagues in ... binding of the plasma membrane. In previous publications, they described tools to control formation of...the effect to a specific time or location can be critical when, say, the gene is essential during development...isn’t powerful enough, what about altering the chemical composition of the membrane itself? The Baskin...Baskin Lab previously published OptoPLD, an optogenetically controlled phospholipase D (PLD). Now they present...

...people who are data oriented, scientifically minded, and able to communicate complex information - all traits... incredibly important to the stability of the biological research enterprise going forward, in this post...the FOR website, the FOR Boston 2015 organizers indicate that the main question they wanted to focus on...Publishing Panel brought in editors from a variety of publications as well as young PIs and all gave advice on ...blogging, write reviews, or write for a university publication, improving your general writing skills will help...academic publishing goals and make you a better communicator overall. Don’t Write in a Vacuum Your research...cognizant of this during my PhD. Submitting a publication doesn’t have to be like throwing it into a black...

...non-viral methods including both chemical and physical methods in the lab. Chemicals like calcium phosphate and...Scientists can also use electroporation, the application of an electrical charge to cells, to increase cell membrane...take up DNA, they can be made competent through chemical manipulation in the lab. This is commonly done... properties. The phagemids are packaged into replication-incompetent phage particles with assistance from... found on an episome, a piece of DNA that can replicate on its own or be integrated within a bacterial...easily cross the negatively charged cell membrane. Physical methods such as electroporation or microinjection...cells. Electroporation on the other hand uses electrical pulses to create transient pores in the cell ...

...potential applications. Challenges of existing mutagenesis methods Several continuous diversification methods... challenging for users to achieve targeted diversification of all nucleotides within a defined region ...Chang Lab have created methods that introduce diversification by targeting a site of interest with dCas9 ...level of variation that can be introduced.   Diversification is limited due to the integration of oligonucleotide...increasing the standard mutation rate seen during DNA replication by 120-fold over background. Due to its modular...only ~2-fold over nCas9 levels. Two sets of modifications were also made to PolI3M to increase the mutation...more than one mutation near the target site. Applications of EvolvR The Dueber Lab used EvolvR to discover...

...tools from across the biological spectrum to learn more about the brain and neurological disease. I was surprised...and, like many biological fields the number potential of experiments enabled by physical, software, and...the things that popped out to me as a novice and indicate why other neuroscience newbies might want to get...brain functions and vice versa. Even within the biological sciences, there’s plenty of interaction between... microbial DNA they all seem to require some mathematical know-how for analysis. If you’re looking to ...presentation area, I honed in on projects using biological tools. In addition to the researcher using sequencing...but are afraid you don’t have the right kind of biological expertise to get into the field, there’s likely...

... given prokaryote typically has genes encoding one or a few restriction modification systems containing...methylation sites are not specifically associated with any restriction modification systems, their sequences... one half of naturally occurring restriction modification systems that prokaryotes use to protect themselves... phage for instance). Aside from restriction modification systems, DNA methylation also plays an integral...integral role in regulating genome replication, repairing mismatched basepairs or small indels that occur...methylases) are independent from the restriction modification systems, yet can still affect whether certain... These specialized E. coli strains have been specifically engineered to be Dam and Dcm methylase-deficient...

...suggesting that QKI is a host protein critical to Zika replication. Human genetic disease. RaPID can also...methods Not in a native context. While native purification preserves RNA structure and naturally occurring...present in the cell, it can also introduce non-physiological RNA-protein interactions due to sample processing...t be used to study endogenous RNAs at their physiological expression levels; however, knock-in of BoxB...used the Contamination Repository for Affinity Purification (CRAPome), to identify highly biotinylated background...research groups that performed BirA*-based affinity purification mass spec experiments. These background proteins...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...