We narrowed to 847 results for: CAL;
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TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m.... the size of other Cas proteins found in the so-called class 2 CRISPR-Cas systems. There are 24 variants... be a fantastic add-on to a diagnostic toolkit, called DETECTR, which has been created by the authors....
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Science Communication Snapshot: DayCon 2015
TypeBlog PostPublishedJune 17, 2015, 9:04 p.m....SITN), we recently sponsored a first-time event called DayCon. DayCon is a one-day conference aimed at...Bio-Medley) to showcase SITN’s strength in the biomedical sciences and decided to offer a varied format... -
Designer PUF Proteins for Any RNA Target
TypeBlog PostPublishedJune 17, 2014, 7:57 p.m....demonstrated in a report published in The Journal of Biological Engineering in March is fused to a translational...that she hopes will change the intracellular localization of RNA. “You can think of changing bases to ... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m....tools. The GCaMP5 and GCaMP6 series of genetically encoded calcium indicators were the hot technology of... -
Management for Scientists: Seeking Feedback
TypeBlog PostPublishedJan. 8, 2015, 2:34 p.m....what is going on and can contribute. Members periodically break, go exploring outside the team, and bring... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m....Annotation of genes in immune cells typically involves the creation of germline knockout mice, which ...Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in improving CD8+ T cell... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog PostPublishedMay 7, 2019, 12:39 p.m....responsible for cancer from genomics data, and large-scale patient cohort data from the MD Anderson Cancer ... wild-type genes were constructed as well. They called this library the CTD2 FASMIC Plasmid Collection... -
SciComm with the Experts at Science in the News Part 2
TypeBlog PostPublishedApril 27, 2017, 2:30 p.m....communication student group and get more involved with your local community. What do Vini and Amy say is the quickest... can grab a beer and chat about their work at a local bar. It doesn't have to be crazy complicated! Listen... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog PostPublishedJuly 3, 2014, 3:48 p.m....there, you might have even seen me bust a move to “Call Me Maybe.” After cocktail hour, we feasted on sliders...portion of the entire night was when Melina and Ken called their parents up to the podium to be recognized... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog PostPublishedMay 12, 2020, 1:15 p.m....match up scientists across fields with different technical knowledge that could help fight COVID-19. They...together, we can create a significant impact on both clinical care and public health for this current COVID-... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog PostPublishedDec. 5, 2019, 1:49 p.m....search bar is now front and center, with a clear call to action - Search! Along with this we’ve also made.... With these three main values we set out to radically redesign our homepage to reflect this message.... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....relationships as part of the job application process can dramatically increase the chances of being seriously considered...you are qualified and why you are interested specifically in the position. We frequently receive clearly... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....incredibly useful molecular biology tool, but like any biological system, it has certain drawbacks. First, the ...Chinese hamster ovary (CHO) cells, as well as in cortical neurons in layer 2/3 of the mouse brain. Rubin... -
Antibodies 101: Fc Effector Functions
TypeBlog PostPublishedMay 18, 2023, 1:15 p.m.... (ADCD) Antibodies can bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent...antibodies on a pathogen activates the immune system's classical complement pathway, starting a cascade of events... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog PostPublishedMay 2, 2023, 1 p.m....throughout an organism’s genome. These reporters have historically been used extensively in fly, mouse, and yeast.... These are effective tools but neglect some biologically relevant situations. Tissue-specific genes... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m....any DNA editing functionality Collecting preclinical and clinical data about a disease or therapeutic Side-by-side... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....could be used in high throughput screening for agrochemical compounds that have the potential to regulate...and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments... -
Fostering communication in remote collaborations
TypeBlog PostPublishedApril 21, 2020, 1:14 p.m....virtual face-to-face meetings as opposed to phone calls most closely replicates how you’d interact with ...investigate my protein of interest in a French clinical cohort, neither of which I would have been able... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....host range and copy number of the plasmid) and typically include a gene that is advantageous for survival...Plasmids 101: Luciferase Monitor the level of a chemical in a particular environment Produce enzymes that... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog PostPublishedAug. 22, 2019, 12:43 p.m....complements the auxotrophy (i.e., synthesizes the critical amino acid). Many common E. coli strains used ...its toxic effect. Negative selection is sometimes called counterselection. Toxin-antitoxin systems One ...