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TypeBlog PostPublishedDec. 14, 2020, 2:15 p.m....Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled receptor genes from the...biosensors Resources on Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors...
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An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog PostPublishedJune 29, 2023, 7 p.m....time for me to complete an online social media certification on social media data, and I was given a fantastic... at Northeastern and Addgene co-op studying communications, marketing, and global fashion studies. She... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....put into them in those later stages. Target identification Not all diseases are fully characterized or...simple pathology. In these cases, preliminary identification of potential targets by CRISPR can accelerate... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....problems, develop general technical proficiency, communicate your work, maintain ethical standards and interact...websites listing alumi and it is easy to use publication records, university websites, ResearchGate and... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based...and mature HA-tagged proteins in vivo. Nature Communications, 10(1). PubMed PMID: 31270320. PubMed Central... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m....be a time when you need to speak publicly to communicate your work. One of my 10 tips for giving a great...; Graduate Student Funding Opportunities Open Education Database: 100 Places to Find Funding For Your ... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m.... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in...Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....published, knockout validated, and work in your application. But…they are the same isotype and therefore ...are identical for antibodies within certain classification groups called isotypes. Why does isotype matter... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....in @nchembio Title: Bye bye L-arabinose drive. Dedicated to Edo, Armin and @KhammashLab. I hope you all...reached the level of expression needed for most applications. Figure 1: With blue light, the VVD... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....launching a company without partners. You need a dedicated team that you know well and trust. This will ...Chowdhury and Clinton Buell, who have very diverse educational backgrounds (an essential component to any successful... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m.... interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease...library of characterized aptamers and supporting publication: http://www.freebase.com/base/aptamer Cruz-... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog PostPublishedMarch 6, 2014, 7:12 p.m....will you meet? How does the Mentor prefer to communicate between meetings (email, phone, etc.)? Make agendas...skills Support inclusion, diversity, and open communication Feel valued by giving back Final tips for success... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m....brain, which has humanistic and philosophical implications. As an example, a group at Caltech used an optical...a handle on some of these mysteries. Another application is clinical; can we find ways to treat patients... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m....generation when bound by the dL5** FAP. The iodine-modifications also shift MG-2I’s excitation wavelength to ...FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation with MG-2I-dL5... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...lines. In the meantime, be sure to browse the publication references and web links from my first post and... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....smaller fragments that are then barcoded for identification. This mixture of barcoded fragments is known...of Toronto. He is an author of 20+ scientific publications and patents. ... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar agreements...portal automates a lot of the process, from notifications and emails to generating the UBMTA link for ... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....represent the fraction of that total with the indicated function. One huge reason CRISPR has become such...achieving the gRNA representation recommended in the publications associated with these libraries. Please note... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....sometimes we don't." - Jennifer Tsang, Science Communications and Marketing Coordinator Non-science favorites...times." - Aliyah Weinstein, Marketing and Communications Manager Addgene's book club Indeed, reading... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....plasmids at Addgene Opperman J et al., Nature Communications, 2019. PubMed PMID: 31346176. Using LADL to...light-activated-dynamic-looping (LADL) system. The group fused dCas9 to a truncated CIB1 protein from Arabidopsis (CIBN), which they...