We narrowed to 809 results for: LAS
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TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....course, UWE Bristol offers an intensive 4-day Masterclass in science communication. The National Co-Ordinating...materials and assignments. 9) Citizen science projects Lastly, if you'd like to directly involve the public in...
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Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m.... create fusions with a gene of interest. These plasmids will be most useful for constructing intermolecular...PMID: 27475975. 6. Gayrard, Charlène, and Borghi, Nicolas. "FRET-based molecular tension microscopy." Methods... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m.... Addgene’s experience helping scientists share plasmids, especially those of the CRISPR variety (see slides...is in one of the top 10 countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....until now. Alice Ting’s lab has developed a new, plasmid-based biosensor to visualise changes in histone...Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse More Fluorescent Protein Biosensors... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....base pair may not be enough to prevent re-binding. Lastly, the sequence inserted by HDR may inadvertently...software Resources on Addgene.org Find CRISPR plasmids and resources Download the CRISPR eBook Read our... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....reference of various fly phenotypes, check out the Classical Genetics Simulator website. One interesting thing...Drosophila CRISPR Resources Deposit Your Drosophila Plasmids ... -
PCR Applications for Copy Number Variation Assays
TypeBlog PostPublishedApril 26, 2022, 1:15 p.m....methods for the determination of amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1... RJ, Paterson ES, Smith ML. The copy-number of plasmids and other genetic elements can be determined by... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m.... The most obvious workaround is to use a multi-plasmid system, where you have one AAV vector dedicated...interrogate immune cells to improve immunotherapy in glioblastoma, and generate programmable screening platforms... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....community. I even spray painted a small erlenmeyer flask with gold glitter spray paint to make a trophy for...Career Posts Resources on the Addgene.org Find Plasmids for Your Research Learn About A Variety of Different... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....on gene transfer, from transgenes to plants, chloroplast to the nucleus, or pathogens to their hosts. ...Quick, Versatile Plant Transgenesis with GreenGate Plasmids ... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (like ...Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References Cullen, B. R. (... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....population of stem cells, usually housed in a single flask. Through a bit of stochastic magic and Poisson statistics...Genome Edit Resources on Addgene.org Find CRISPR Plasmids for Your Research Check out Our CRISPR Guide Find... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....instead of three, and we are sending hundreds of plasmid requests out each day. As you may imagine, it keeps...together.I realize I may have painted a rose-colored-glasses-esque picture of how everyone is kind and collaborative... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....Favorable conditions - Expansion of networks The last stage is Exponential Growth, named after the growth...has worked in molecular and microbiology for the last two decades as a staff scientist, but before going... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m.... development includes everything from tracking plasmids from the moment a deposit kit is sent to a depositor...improvements, you are benefited with reassurance your plasmids will not get lost in a freezer and your viral ... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....similar on-targeting cutting as full-length gRNAs Lastly, the effects of guide length on off-target cuts...on Addgene.org Check out this guide to pooled plasmid libraries Find all of the CRISPR Pooled Libraries... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....PHP.B permissive phenotype in mice. They took a classic genetics approach and interbred C57BL/6J and BALB...permissive and non-permissive mouse strains. PHP.B plasmids and PHP.eB viral preps are available from Addgene... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog PostPublishedMarch 12, 2019, 1:37 p.m....and a room of 5-30 faculty members. The session lasts up to an hour. You will get 5-10 minutes to present...quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m.... from injury, this protein leaks out into the cytoplasm, migrates out of the cell, and binds cells that...Library Can be Used to Study Cancer Drug Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana...