We narrowed to 792 results for: LAT
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TypeBlog PostPublishedApril 1, 2025, 1:15 p.m....to repair the DNA using the edited strand as a template — in this case, encouraging it to incorporate ...Like other transversion editors, this editor had relatively high indel rates ranging from 4.7% to 30% at ...
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Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....and experimental needs. Since its invention in the late 1990s, Gateway cloning technology has become very...Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR to Quickly... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....session is to start with something completely unrelated to the issue and consider how it could be shaped...problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....feasible, it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged...for Cas9. However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....applications, they have become indispensable tools for manipulating cells and organisms. They can be used for a ...This is because most titering methods rely on isolating genomic DNA from transduced cells and quantifying... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m.... require sequencing of many samples at once for later comparison. These next-generation sequencing techniques...technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move ... -
Transferable Skills Guide: Conflict Resolution
TypeBlog PostPublishedJan. 4, 2022, 2:15 p.m....different opinions, and some of them would acknowledge later that without my “neutral” presence to alleviate ... that nobody is really owning. Conflict in this latter scenario usually manifests as the assumption “I... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog PostPublishedOct. 5, 2021, 1:15 p.m....tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular... Originally reported as a monomer16, IFP2.0 was later found to be a dimer3,15. ND-not determined in parallel... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m....super-resolution microscopy techniques, such as Stimulated Emission Depletion (STED), and Stochastic Optical... expand the self-labeling capabilities of this platform. The Janelia Fluor ligands provide brighter and... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog PostPublishedMarch 25, 2025, 1:15 p.m....replaces a target region with a newly synthesized, templated sequence. And the story still isn’t over! Keep...Base Editor A base editor that relies on DNA glycosylation to create an abasic site. In this post, it refers... -
New Antibody Animation!
TypeBlog PostPublishedFeb. 22, 2024, 3:13 p.m....Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ... -
New Coomassie Protocol Video
TypeBlog PostPublishedOct. 31, 2023, 1:15 p.m....determine your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant... -
New Antibodies in the Lab Animation!
TypeBlog PostPublishedNov. 19, 2024, 2:15 p.m....where to start or what your options are? Addgene's latest animation is here to help! "Antibodies 101: Antibodies... -
Plasmids 101 eBook 4th Edition
TypeBlog PostPublishedSept. 5, 2023, 1:15 p.m....101 covers everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog PostPublishedJuly 11, 2024, 1:15 p.m....We are very excited to share the latest edition of our Viral Vectors 101 eBook! This practical guide... -
Antibodies 101 Series on TikTok
TypeBlog PostPublishedMarch 19, 2024, 1:15 p.m....whenever a new video is posted! If TikTok isn't your platform, all of our videos will also be shared on our ... -
A Case Study of Addgene Bioinformatics
TypeBlog PostPublishedMay 25, 2023, 1:15 p.m...., they break down the unique challenges of our platform and how we were able to address these issues using... -
'Tis the Season to #DeckTheLab
TypeBlog PostPublishedDec. 2, 2016, 3:30 p.m....the month of December are eligible to win a hot chocolate party that will warm up the entire lab. We’ll ... -
The 12 Days of CRISPR: 2021
TypeBlog PostPublishedDec. 28, 2021, 2:15 p.m....to me: enhanced prime editing systems through manipulating MMR machinery. On the fifth day of CRISPR, Addgene... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog PostPublishedDec. 5, 2023, 2:15 p.m....Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers: Atharva Sahasrabudhe...