We narrowed to 708 results for: MAK;
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TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....functional before investing time and resources into making a stable cell line. When expressing a viral construct...
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Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....bbdone with our bbduk process. At first, to make sure we understood the concept of channels and implemented... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....work stalls. We become so fixated with the idea of making something the best it can be that it seems impossible... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m.... and high specificity of anti-epitope antibodies make this approach appealing, but the disadvantages must... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....retrograde direction from the synapse to the cell body, making it a great tool to study neuronal connectivity.... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....t simply rely on chance (or random diffusion) to make this happen. There are several different versions... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....often looks like the graph on the left, it might make more sense once you realize events are plotted on... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m.... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....gene! STEP 2: Generate an Expression Clone When making the expression clone, it is important to choose... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m.... long latency period, and rarity of mesothelioma make it different from many other types of cancer. Mesothelioma... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....engineering has revolutionized the gene editing field by making experimental workflows considerably easier, faster... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m....through smart engineering Different FLP versions – making FLP efficient The first version of FLP discovered... -
5 Reasons to Use Reddit for Science Communication
TypeBlog PostPublishedOct. 20, 2016, 2:30 p.m....until 5 pm). The ability to see the questions first makes Reddit a great platform for those who aren’t so ... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m.... than engineering an entirely new protein. This makes it much cheaper to use Cas9 activators. This larger... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....//doi.org/10.1111/1574-6968.12241 Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved... -
To Codon Optimize or Not: That is the Question
TypeBlog PostPublishedNov. 12, 2020, 2:15 p.m....organism's codon bias. The idea is that scientists can make mutations throughout a gene of interest based on... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m....gRNA targets in that region. The CRISPR Software Matchmaker can be used to determine the pros and cons of... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m.... generally ensure cell line identity and purity making it easier for comparable studies to be performed... -
Getting the Most from Your Lentiviral Transduction
TypeBlog PostPublishedApril 7, 2016, 2:30 p.m....physiology of different cell types, however, can make this assumption incorrect. One of the earliest steps... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....reproducibility controls". Biological replicates are what makes up your sample size (aka your n value)" and should...