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Showing: 661 - 680 of 701 results

  1. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted...

  2. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...//doi.org/10.1111/1574-6968.12241  Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved...

  3. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...often looks like the graph on the left, it might make more sense once you realize events are plotted on...

  5. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...t simply rely on chance (or random diffusion) to make this happen.  There are several different versions...

  6. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...bbdone with our bbduk process.    At first, to make sure we understood the concept of channels and implemented...

  7. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...work stalls. We become so fixated with the idea of making something the best it can be that it seems impossible...

  8. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ... and high specificity of anti-epitope antibodies make this approach appealing, but the disadvantages must...

  9. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...retrograde direction from the synapse to the cell body, making it a great tool to study neuronal connectivity....

  10. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ... than engineering an entirely new protein. This makes it much cheaper to use Cas9 activators. This larger...

  11. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...gRNA targets in that region. The CRISPR Software Matchmaker can be used to determine the pros and cons of...

  12. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... generally ensure cell line identity and purity making it easier for comparable studies to be performed...

  14. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...reproducibility controls". Biological replicates are what makes up your sample size (aka your n value)" and should...

  18. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...exposed side chain that can be easily manipulated to make reactive species without affecting the avidin binding...

  19. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...gating uses the same color for different markers, making it easy to gate on the negative cell population...
Showing: 661 - 680 of 701 results