We narrowed to 794 results for: Mpl

... understand the process, we’ll walk through an example experiment where we might use Gateway cloning to...into destination vectors. The cloning process is simple -  no restriction, ligation or gel purification...

...editing in squid is possible nonetheless - the first example was of a pigmentation gene.   Genus Microtus (Zoe...Donaldson) Voles have fascinating social behavior. For example, two similar species have very different social...

...light inducible ion channels (plasmid # 20298 for example) have revolutionized Neuroscience. They are the...within neuronal networks in living animals. To accomplish such cell specific manipulations in vivo, FLEx...

...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the...Cre not only halted the neurological damage but completely reversed it, regardless of age or symptom severity...

...avoid rousing the trolls. 3. The AMA process was simple and well organized. You can check out the r/Science...posters (this advice included leaving out the term “implicit bias” which is apparently a troll trigger phrase...

... purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag, a small molecule...substitution. Alternatively, you can express the completely wild type protein from the same plasmid.  Upon...

...regularly. Dystrophin and genome editing Since complex oligonucleotide treatment comes with many challenges...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...

...specific list of the candidate off-target sites. For example, if there are 10 putative off-target locations ...confounding your results should be minimal. For example, if your goal is to generate a knock-out cell line...

...epigenetic modifications across the genome) has been implicated in disease states, and targeting the epigenome...more frequently inherited by daughter cells. For example, KRAB induced silencing was transient and quickly...

...emotions and assumptions. I’m not just talking about simple things like “Which route should I take to get to...choosing a training lab, I strongly suggest you complete a work rotation in the lab if at all possible....

...the cell to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of...repairs the DNA using the U-containing strand as a template, copying the base edit. Figure 2: Cytidine...

...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

...vectors, but what we lacked were videos guiding completely new members of the lab or those taking introductory...

...executing, and troubleshooting your experiments. For example, if you’re very new to antibodies as research tools...

...sharing In a recent review on CRISPR technology, for example, the authors Patrick Hsu, Eric Lander, and Feng...

...Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with NEB's DADExperts: Matthew...

...festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https...

...ASCBEMBO19 #MCDB 🎄🎅🎁🐸🐸🐸🐸 pic.twitter.com/2MPLawgNQl — Jennifer Landino (@jenelandino) December 3,...

... of the best ways to learn about a new field is simply to know where to look and how to start. There is...

...information updated, export controls and regulations are complex and may change suddenly. Please email [email protected]...