We narrowed to 729 results for: NSI;
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TypeBlog PostPublishedMarch 30, 2017, 2:30 p.m....commonly employed mismatch nuclease assays. Sensitivity: Less than 20 ng of total gDNA is sufficient ...
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Hot Plasmids - May 2022
TypeBlog PostPublishedMay 26, 2022, 1:15 p.m.... live imaging application. Each visual barcode consists of a fluorescent protein coupled to a specific... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....Protein Ladder by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective... -
Viral Vectors 101: Viral Vector Elements
TypeBlog PostPublishedJuly 25, 2023, 1 p.m....packaging of the virus into particles. E2A/E2B are responsible for viral replication, with E2B being the primary... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....questions to be clarified, especially with regard to licensing models. Direct sharing of work from universities... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m....unsure what amount of antibody to use, you should consider using an isotype control. Pro tip! Many companies... -
Plasmids 101: Blue-white Screening
TypeBlog PostPublishedJune 4, 2015, 1:03 p.m....making your plates: X-gal is light and temperature sensitive and needs to be added to media after autoclaving... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit the number... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m.... activator-like effector nuclease (TALEN) kit consisting of 834 plasmids. The researchers showed they ... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....samples you may have, access to one or more large, expensive ultracentrifuge. Figure 1: An outline of... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....Russell, P., & Guttman, M. (2014). Methods for comprehensive experimental identification of RNA-protein interactions... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....or Jay Bradner ([email protected]). Considerations when getting the dTAG system up and running... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....genes of interest. These transcription factors consist of the GAL4 or ZIF268 DNA binding domains fused... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m.... new growth phase of the organization and the expansion of our impact, I’d like to take a moment and honor... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....exonic gRNAs. In fact, the method is specific and sensitive enough to detect gene copy number based on the... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....salary information as of December 1st. Salary inconsistencies across geographical regions When we originally... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....funding bodies and review committees encourage and consider preprints as demonstration of productivity. Part... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....resources. There are three types of cognitive load: intrinsic cognitive load is the effort associated with a... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....did not just automatically come to a solution. Consider the thoughts that went through your head as you... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....Lerner RA, Wigler M (1988) Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae...