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We narrowed to 847 results for: PLE

Showing: 661 - 680 of 847 results

  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...are working with proto-oncogenes or with human samples that have not been tested for HIV.  If not using...must be chosen carefully. Often researchers want a simple set-up where the gene of interest is expressed ...experience in transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly...

  2. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... viral DNA sequences and expression profile at multiple steps from plasmid amplification in bacteria to... set of injections into your animal model. For example, in a recent study by the Wickersham lab (Lavin...essential that every new vector, whether it be a completely new transgene or merely a new lot, be thoroughly...

  3. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...cassette (Dickinson et al., 2015) made this step as simple as heat-shocking to induce the expression of a ...direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates which reduces...worms are perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted...

  4. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...extracting the DNA from fossilized amber and supplementing it with the DNA of present-day frogs via genetic...template.  Each zinc finger recognizes a nucleotide triplet and when several ZFNs are strung together, it can...the ZFNs, but instead of recognizing nucleotide triplets, the components recognize individual nucleotides...

  5. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...CRISPR-based gene editing (see table below). For example, the far-red light-activated split-Cas9 (FAST) ...translocation of the protein BldD to the nucleus. To couple this light-dependent activity to transcription,...proteins by fusing Cas9 to photoreceptors. One prime example was from the lab of Michael Lin, who developed ...

  6. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ... Denise Roche, to edit the translation. We are pleased to announce that CRISPR 101: Una herramienta de...repository. This incredible gift will allow more people to access helpful resources for their CRISPR experiments...

  7. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with NEB's DADExperts: Matthew...KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv ...

  8. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...pm. If you want to learn about, and practice, principles of clear, accessible writing through the lens...Poster Session TipsHow to Make Friends and Meet People at a Scientific Conference New to SfN? Try A Novice's...

  9. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ... AAV performed in that experimental setup. For example, this is an experiment using the chemogenetic tool...thank you for your contribution to open science.  Please note that the AAV Data Hub is a beta project, and...

  10. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...was designed simply to be stable and novel, the principles that created it have been applied to numerous...protein evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools...

  12. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...Sleigh bells, please! It's time to announce the winners of the 2022 #DeckTheLab contest!  and it's.....I was bored this weekend while waiting to take samples from my assay at like 23:00, so I decided to make...

  13. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...Blog is a great way to get your name out there! Completed blog posts are also great to include on CVs and... with and discuss your work with many different people. Recent Guest Writers Have Included: Wenning Qin...

  14. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...basics - diving into the ins and outs, tackling complex questions, and giving tips for consistently generating... with some behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that a few...

  15. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ..., in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine microenvironment... cells, an important immune cell type, in more complex and physiological microenvironments. These screens...

  16. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ... (AAVrg), and volumes of 1 mL, 2.5 mL, or 5 mL. Please note that all orders will be shipped in 100 µL ...are a big deal, so if you have questions, we have plenty of information available for you to dig into. Check...

  17. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...just starting in the lab, but they are exactly the people you should talk to if ruminating the trials and... 14:32 - 16:07 : What do you think drives other people to do science? 16:08 - 22:04 : How have the make-up...

  19. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...cultural shifts that can have bigger impacts. For example, if you joined an office where everyone used a ... about this, and even has a list of biological samples that are stored long term at -70 °C. My Green Lab...

  20. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...contributor of careers blog articles, Joanne had plenty of advice for the group of juniors and seniors,...working lab full of busy Addgenies preparing plasmid samples for shipping. This was the favorite part of their...
Showing: 661 - 680 of 847 results