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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 837 results for: RING

Showing: 661 - 680 of 837 results

  2. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...repressing and activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed...donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find ...

  3. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...biology because of an image that was honestly quite boring. My Bio 101 professor displayed a codon table —...mammalian cells like neurons, may not replicate during the course of your experiment, meaning you don’...

  4. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... target guanosine instead, perhaps analogous engineering of UNG, which excises uracil, could allow it ...considerably. And there’s so much more exciting protein engineering going on in the base editing field! From CRISPR-free...

  5. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...inducible promoters with different degrees of stringency and inducibility (Toh et al. 2023), and additional...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit...

  6. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...blog post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient...Frank et al. 2003. “A Directional Strategy for Monitoring Cre-Mediated Recombination at the Cellular Level...

  7. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...Vader in "Return of the Jedi" While Vader was referring to the evil side of a mystical "Force," this quote...posttranslational modifications can disrupt FP folding rendering the protein non-fluorescent. Unfortunately, accumulated...

  8. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...the plasmid without the independent variable. Referring back to the experiment associated with Figure ...aliquot/preparation. The replicate control isn’t measuring or assessing reproducibility of the effect of ...

  9. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...high protein levels including:  ompT: Strains harboring this mutation are deficient in outer membrane ...recommended as these auxillary plasmids may be isolated during the prepping process. How does inducible expression...

  10. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This effort is two-fold... “politicized science” ABC News: White House considering gutting 38 EPA programs: draft budget  Science...

  11. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...sequence detection method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127... Detection of SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159...

  12. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...vivo probes, due to low autofluorescence, low scattering, and deep penetration of red-shifted light. The...about cellular events via  fluorescence changes occurring due to protein synthesis, degradation, or reconstitution...

  14. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...tribulations of the process, I’ve provided guidance and mentoring to several people that found my insights helpful...'s about how YOU will fit into a department and bring in grants. What kind of colleague will you be? Do...

  15. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...donor, you can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This...Concordet J-P. Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection...

  17. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...seems to be the case that there is no universal scoring system for selecting a gRNA, as the method of producing... is taken in the interpretation of results by requiring multiple sequences targeting a gene to score in...

  18. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...transferable skills while working in the lab and by offering advice on areas where you might need some help...Scientists: Managing v Leading Gain Management Skills Volunteering at a Professional Organization Resources on...

  19. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...tracing techniques: exploiting the offspring of genetic engineering." Journal of neuroscience methods ...

  20. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...looking for, but there are also benefits gained by lowering the complexity of the screen. For example, the..., Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....
Showing: 661 - 680 of 837 results