We narrowed to 849 results for: RING

...expression baseline. Copy number is then determined by comparing results to a standard curve. For CNV assays, it...gives an absolute value of DNA concentration. By comparing the number of positive droplets for the gene of...

...mouse knockout lines should increase. New genome engineering tools also offer hope for the creation of new...knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures were ...

...FPs that dimerize can form dynamic contacts that bring apposing membranes together and can wrap the membranes...reporter that undergoes a conformational change to bring two FPs on a single protein closer together or further...

...similar resource for those involved in monocot engineering projects. The MoClo tools will work in any dicot...think plant biology is massively underfunded considering how important it is. I would really like to reach...

...to get into the same cell. For simple cloning requiring just one or two recombination events, homemade...DNA polymerase This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your ...

...step of the series is consistent at 1:2. When preparing a dilution series, use a new pipette tip for each...components can affect the final readout. When preparing a standard curve, change pipette tips and mix ...

...community is a kind of duty for researchers when considering the peer-review system of journals and grant ...technologies developed abroad, so it's a little boring. I think that such tendency is one of the reasons...

...Neighborhood.   Work/life balance. Is it truly possible to bring all aspects of our lives under control? Balance ... friends. If there is any guilt, you should be sharing it absolutely equally, which means you only have...

...blog post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient...Frank et al. 2003. “A Directional Strategy for Monitoring Cre-Mediated Recombination at the Cellular Level...

...about. Instead of reading a paper and getting my bearings, I would read a paper, panic, and then download...think the field is going or what areas are worth exploring.  Edit and rewrite, then repeat If you’re like...

...full-length (19-20 bp) or short (17-18 bp) guides. When comparing the toxicity of guides that have 1-bp mismatch...Central PMCID: PMC3988262. 4. Hart, Traver, et al. "Measuring error rates in genomic perturbation screens: gold...

...pinhead—contribute to the “sculpting” of tissues and organs during embryo development. Single cell sequencing-based...Single-cell reconstruction of developmental trajectories during zebrafish embryogenesis." Science 360.6392 (2018...

...inducible promoters with different degrees of stringency and inducibility (Toh et al. 2023), and additional...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit...

...repressing and activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed...donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find ...

... children, it can also be applied to research. Sharing knowledge and tools is the best way to help propel...tools, and resources. Luckily for us, they like sharing! You can find data, analysis tools, and other scientific...

...biology because of an image that was honestly quite boring. My Bio 101 professor displayed a codon table —...mammalian cells like neurons, may not replicate during the course of your experiment, meaning you don’...

... target guanosine instead, perhaps analogous engineering of UNG, which excises uracil, could allow it ...considerably. And there’s so much more exciting protein engineering going on in the base editing field! From CRISPR-free...

...mesothelioma. Asbestos, and certain other naturally occurring asbestiform minerals such as erionite, are the...an immediate stop to the use of asbestos, thus ensuring that future generations will be protected from...

...Vader in "Return of the Jedi" While Vader was referring to the evil side of a mystical "Force," this quote...posttranslational modifications can disrupt FP folding rendering the protein non-fluorescent. Unfortunately, accumulated...

...the plasmid without the independent variable. Referring back to the experiment associated with Figure ...aliquot/preparation. The replicate control isn’t measuring or assessing reproducibility of the effect of ...