Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 846 results for: RING;

Showing: 661 - 680 of 846 results

  1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...similar resource for those involved in monocot engineering projects. The MoClo tools will work in any dicot...think plant biology is massively underfunded considering how important it is. I would really like to reach...

  2. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...to get into the same cell. For simple cloning requiring just one or two recombination events, homemade...DNA polymerase This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your ...

  3. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...step of the series is consistent at 1:2. When preparing a dilution series, use a new pipette tip for each...components can affect the final readout. When preparing a standard curve, change pipette tips and mix ...

  4. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...community is a kind of duty for researchers when considering the peer-review system of journals and grant ...technologies developed abroad, so it's a little boring. I think that such tendency is one of the reasons...

  5. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...Neighborhood.   Work/life balance. Is it truly possible to bring all aspects of our lives under control? Balance ... friends. If there is any guilt, you should be sharing it absolutely equally, which means you only have...

  8. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...about. Instead of reading a paper and getting my bearings, I would read a paper, panic, and then download...think the field is going or what areas are worth exploring.  Edit and rewrite, then repeat If you’re like...

  9. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...mesothelioma. Asbestos, and certain other naturally occurring asbestiform minerals such as erionite, are the...an immediate stop to the use of asbestos, thus ensuring that future generations will be protected from...

  10. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...biology because of an image that was honestly quite boring. My Bio 101 professor displayed a codon table —...mammalian cells like neurons, may not replicate during the course of your experiment, meaning you don’...

  11. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... target guanosine instead, perhaps analogous engineering of UNG, which excises uracil, could allow it ...considerably. And there’s so much more exciting protein engineering going on in the base editing field! From CRISPR-free...

  12. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...inducible promoters with different degrees of stringency and inducibility (Toh et al. 2023), and additional...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit...

  14. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...repressing and activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed...donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find ...

  15. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...blog post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient...Frank et al. 2003. “A Directional Strategy for Monitoring Cre-Mediated Recombination at the Cellular Level...

  16. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...Vader in "Return of the Jedi" While Vader was referring to the evil side of a mystical "Force," this quote...posttranslational modifications can disrupt FP folding rendering the protein non-fluorescent. Unfortunately, accumulated...

  17. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...the plasmid without the independent variable. Referring back to the experiment associated with Figure ...aliquot/preparation. The replicate control isn’t measuring or assessing reproducibility of the effect of ...

  18. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...high protein levels including:  ompT: Strains harboring this mutation are deficient in outer membrane ...recommended as these auxillary plasmids may be isolated during the prepping process. How does inducible expression...

  19. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This effort is two-fold... “politicized science” ABC News: White House considering gutting 38 EPA programs: draft budget  Science...
Showing: 661 - 680 of 846 results