We narrowed to 868 results for: TOR

...is correct. It's also best to use 1-2 ug of the vector for digestion. PCR Design primers that overlap ... probably left your plate for too long in the incubator. The small colonies are called satellite colonies...after the screening, sequence the plasmid. Many vectors contain sequences that are complementary to standard...

...team and software engineers to update our Lab Inventory Management System (LIMS) to accommodate our new... discussed them in meetings and 2) creating a pictorial guide for our engineers (Fig. 2). Creating this... a lot on context. New people in the lab or collaborators will not have the context you do, which means...

...way they are, the research antibodies in your refrigerator often need a little help to be useful. Mainly...of e-Amino Groups Available for Conjugation of Effector Molecules to Monoclonal Antibodies. Hybridoma ...resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies 101: The Basics of Western...

...Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse protein ...GBPs together to create a complete transcription factor, activating luciferase transcription. Tang et al...delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is ...

...aligns with your work, your advisor may want to mentor you during the grant application process. When ...first. Additionally, reach out to peers and collaborators who have succeeded at grant writing to see what...write your first grant proposal" by Soapbox Science Editor in Nature Blogs "How to write a research project...

...They play important roles in many cellular and regulatory processes, but much of their functionality is...effects can be minimized by disrupting lncRNA regulatory elements that are crucial for transcription and..., on the other hand, recruits transcriptional activators such as VP64, SAM, or SunTag to specific ncRNA...

...NG  - SpRY nuclease  C to T Base Editor SpRY A to G Base Editor SpRY   NYN/NRN NYN is less effective...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

...improved search algorithm which was trained on key factors from past PubMed user searches, such as clicks ...other areas of science. Scientists often post tweetorials, or threaded summaries, of their papers. These...publications in the fields of research they support. Monitoring these types of accounts can help you find papers...

...Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some ...particular tool has gained widespread popularity – and notoriety – as a tool of choice in the field: dCas9 fused...Daniel M. Sapozhnikov is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working...

...2019; Chen & Liu, 2023). This method uses a prime editor (typically a Cas9 H840A nickase fused to a reverse...optimization efforts. This holds true for any prime editor experimentalist, and especially shines once large-scale...Collection The CRISPR Guide Empty gRNA Expression Vectors...

...If you have ever worked in a molecular biology laboratory you have likely done a polymerase chain reaction...method that can help save time and effort in the laboratory.  There are two main categories of multiplex ... Variations Quantifying low abundance targets Monitoring subtle changes in target DNA levels Multiplex...

... practicing with your colleagues, ask them to brainstorm potential questions you may receive. People often...minutes per poster. Ask the presenter to give the “elevator pitch,” of his/her project – kindly let the presenter...Addgene website Become of a member of the Addgene depositor community Learn about careers at Addgene ...

... like PCR, cloning, transfection, or long-term storage. But what about those pesky situations where your...maximize cell density and plasmid yield as these factors are generally, but not perfectly, correlated. ...manufacturer. On Addgene plasmid pages, you may see depositor recommendations to grow a plasmid at 30℃ or room...

...population of gRNA-containing lentiviral transfer vectors, each targeting a specific gene within the genome...gRNAs are then cloned into lentiviral transfer vectors to create the CRISPR library. CRISPR libraries ...library that is right for you There are several factors to consider when selecting a CRISPR library for...

...the sequencing results of the plasmids in our repository with known reference sequences, such as full ...full plasmid sequences provided by the laboratories that deposit their plasmids with us or other entries ...entries in NCBI’s numerous databases. As our repository has grown over the years (we now have over 60,000 plasmids...

...Protein (GFP), a protein from the jellyfish Aequorea Victoria. Since that discovery, the family of FPs just ... used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato...have a filter that will pass blue light to the detector/camera, then BFPs are of no use to you. When using...

... construct. This strategy can be used to build vectors with diverse functions, and thus, is used in many...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...Shetty RP, Endy D, Knight TF. Engineering BioBrick vectors from BioBrick parts. Journal of biological engineering...

...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...Further complicating the issue is the long and ugly history of reporting false essentialist links between biology...actively look for and consider bias as a confounding factor when interpreting findings, particularly when researching...

...colonies. The best way to do this is by using your vector with and without an insert to verify that the primers...cultures of your positive clones. If you want to store your clones longer term, just streak the colonies...PCR primers work in the first place: the backbone vector with and without an insert. These controls are ...

...number of chemical and peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be...purification to get your protein as pure as possible? Vectors that offer different combinations of tags are readily...Protein (GFP) Fluorescent Protein Guide Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his...