We narrowed to 707 results for: ang

1. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

   Type

   Blog Post

   Published

   June 25, 2024, 1:15 p.m.

   ...editing efficiency such as the length of the RTT overhang, the GC content of the primer binding site, and...

2. Plasmids 101: Protein Expression

   Type

   Blog Post

   Published

   June 7, 2018, 1:17 p.m.

   ...technology, plant cells have been used to express a wide range of proteins including antibodies and pharmaceuticals...

3. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   Published

   Jan. 30, 2018, 2:42 p.m.

   ...the feedback, but at least you can start making changes for the best. Positive feedback is equally as important...

4. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   Published

   Aug. 3, 2023, 1:15 p.m.

   ...the markers in the panel. This complexity index ranges from 0-1, with a value of “0” indicating those ...

5. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   ...to K‐Ras and inhibit Sos‐mediated activation." Angewandte Chemie 124.25 (2012): 6244-6247. PubMed PMID:...

6. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   Published

   Sept. 12, 2017, 1:44 p.m.

   ...wanted to see how low we could go. We prepared a range of spiked samples from 0.1-20% and discovered that...

7. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   Published

   Feb. 4, 2016, 3:30 p.m.

   ...The backbone modifications listed can refer to changes made to the original backbone by the depositing...

8. Truncated gRNAs for Regulating Gene Expression

   Type

   Blog Post

   Published

   Jan. 10, 2017, 3:37 p.m.

   ...transcriptional regulator without making any functional changes to Cas9. For instance, when native (nuclease-competent...

9. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   Published

   Aug. 18, 2015, 2:30 p.m.

   ...scaled up for genome-wide screening due to the broad range of potential target sequences and ease of generating...

10. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ... entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988 Aug;62(8):2636-43.) PubMed...

11. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    Published

    June 12, 2014, 3:30 p.m.

    ...to expand the toolkit. We have included a wider range of terminators than are commonly used as we have...

12. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    Published

    Aug. 30, 2022, 1:15 p.m.

    ...red lines), which maintains structural epitopes (orange region), but it can block epitopes hidden within...

13. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    Published

    July 1, 2022, 1:15 p.m.

    ...their science, meaning that they will heavily evangelize for the mode of communication they connect best...

14. Tips for arabidopsis transformation

    Type

    Blog Post

    Published

    Oct. 25, 2018, 1:23 p.m.

    ...Agrobacterium all over your gloves, so be sure to change gloves between constructs if you are transforming...

15. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ...sgRNA targets. CRISPRi is effective in a narrow range around the transcription start site (TSS). The Root...

16. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    ...engineered AAV serotypes that further expands the range of AAV properties is available. While only natural...

17. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ... you’re more of a leader than you thought! When change happens at your organization, when your boss needs...

18. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ... very diverse family of viruses with broad host range and tissue tropism (Borkenhagen et al., 2019). AdVs...

19. Getting to Know Expansion Microscopy

    Type

    Blog Post

    Published

    June 20, 2023, 1:15 p.m.

    ...e73775. https://doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging...

20. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...as a linear polymer of A,T,G, and C nucleotides arranged in a double helix. People are wrong. In reality...