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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 845 results for: ins;

Showing: 661 - 680 of 845 results

  1. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...Sciences program at Harvard University and pick their brains on how they've managed to keep themselves happy...year dip (see graph). Niroshi and Ben give their insights as to what causes this dip. 11:11 - 15:37 : How...

  2. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...submission is used to create a data report, which contains experimental data showing how the antibody performed...immunocytochemistry, ELISA — but in all cases, it contains a detailed description of the experimental conditions...

  3. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...probably bring in a mug or reusable water bottle instead of buying yourself a big stack of plastic cups....could be: Setting up a recycling program at your institute. Set your -80 °C freezer to -70 °C and teach other...

  4. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...protocol has worked well for us for multiple E. coli strains, including DH5alpha, XL1 Blue, TOP10, ccdB survivalTM...competent cells, then you're best off following the instructions that came with them. If you decided to make ...

  5. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...loads of inspiration for the current and the next generation of microbiologists. If you got inspired to produce...

  6. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ... technologies were without controversy, and it remains to be seen how popular any one of them will become... though a little confusing to the uninitiated, contains much of the information needed to understand how...

  8. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...sessions led by scientists, which is hosted on our Instagram channel every Sunday. What's planned for the upcoming...participate in our one-hour Live Q&A session on Instagram every Sunday. Undergraduate and graduate students...

  9. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors...control gene expression in mouse neurons and in the brains of living mice both quickly and significantly. ...

  11. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...Kathleen Ottina and David Sabatini at the Whitehead Institute and MIT, started with a list of 17 signaling pathways...pathways. With support from the National Cancer Institute, they expect to add another 200 or 250 constructs...

  12. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the...ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA Editing...

  13. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...series on Management for scientists. This second installment in the Management for Scientists series will ...Walking Around" – don't hide in your office. Stop by instead of emailing.    Demonstrate you are open to feedback...

  15. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...develop a searchable and sortable datatable that contains validated gRNA sequences. Validated in this context...to deliver your CRISPR components as RNPs (ribonucleoproteins) and therefore usually transcribe your gRNAs...

  16. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...suite of four systems which utilizes custom mouse strains and lentiviral expression vectors (Table 1). The...generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347...

  18. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ... own.” “Someone to act as your ‘cheerleader’ – inspire you.” “Also a mentor can give you realistic expectations...mentor does all of these things and more. It is instructive (and entertaining) to review this list of bad...

  19. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...June, we announced a new partnership with the Institute for Protein Innovation (IPI), in which Addgene.... The first IPI collection, 31 rAbs targeting integrins, will be released early this year.  In September...
Showing: 661 - 680 of 845 results