We narrowed to 852 results for: ins;

...to cut other DNA or RNA sequences. Nucleases: Proteins that bind to gRNAs and are directed by the gRNAs...prime editing and the identification of other Cas proteins continue to expand the targeting range. CRISPR...

...tradition in which we choose our teams and start brainstorming months in advance. Our teams comb the news (...Friday. So if you’re interested in finding out who wins this year’s Addgene costume contest, like us on ...

...academic institutions can afford to hire. It will be difficult for some of these institutions to manage...

... about how fluorophores other than fluorescent proteins can be incredibly useful in FRET experiments. ... our posts if you have any questions or useful insights. If you're interested in blogging for us, fill...

... share their AAV validation data. The Data Hub contains a curated collection of community submissions ...) can save you significant time and money. For instance, this report shows that anterograde, monosynaptic...

...and metabolism. Melina has worked at Genetics Institute in Cambridge, MA and Sequana Therapeutics in San...the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control Addgene's...

...Sciences program at Harvard University and pick their brains on how they've managed to keep themselves happy...year dip (see graph). Niroshi and Ben give their insights as to what causes this dip. 11:11 - 15:37 : How...

...submission is used to create a data report, which contains experimental data showing how the antibody performed...immunocytochemistry, ELISA — but in all cases, it contains a detailed description of the experimental conditions...

...probably bring in a mug or reusable water bottle instead of buying yourself a big stack of plastic cups....could be: Setting up a recycling program at your institute. Set your -80 °C freezer to -70 °C and teach other...

...protocol has worked well for us for multiple E. coli strains, including DH5alpha, XL1 Blue, TOP10, ccdB survivalTM...competent cells, then you're best off following the instructions that came with them. If you decided to make ...

...loads of inspiration for the current and the next generation of microbiologists. If you got inspired to produce...

...Directors brings human resources experience and insight to the now 13 member board at Addgene. Let’s meet...Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog...

...sessions led by scientists, which is hosted on our Instagram channel every Sunday. What's planned for the upcoming...participate in our one-hour Live Q&A session on Instagram every Sunday. Undergraduate and graduate students...

... technologies were without controversy, and it remains to be seen how popular any one of them will become... though a little confusing to the uninitiated, contains much of the information needed to understand how...

...lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors...control gene expression in mouse neurons and in the brains of living mice both quickly and significantly. ...

...the Genetic Perturbation Platform at the Broad Institute, John Doench. A genetic screening project can ...still offering the potential for true biological insight. Pooled and individual plasmid formats A first ...

...Kathleen Ottina and David Sabatini at the Whitehead Institute and MIT, started with a list of 17 signaling pathways...pathways. With support from the National Cancer Institute, they expect to add another 200 or 250 constructs...

...bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the...ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA Editing...

...series on Management for scientists. This second installment in the Management for Scientists series will ...Walking Around" – don't hide in your office. Stop by instead of emailing.    Demonstrate you are open to feedback...

...suite of four systems which utilizes custom mouse strains and lentiviral expression vectors (Table 1). The...generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347...