Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 818 results for: lars

Showing: 661 - 680 of 818 results

  1. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...systems, methyltransferases, methylate DNA at particular sequences to prevent them from being degraded...Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ...

  5. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...  Prevent Interruptions If you need to focus, a large set of headphones can act as a Do Not Disturb sign...would build a wall of pipette tip boxes when she particularly needed to focus, which was extremely effective...

  7. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...assigned a paper to give as a journal club to my molecular biology class. A Cell paper. With six figures....and/or apprehensive. I still get nervous quite regularly, and I've had multiple jobs where all I did was...

  8. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...Moks 1987). These tags, however, tended to be very large and often required the incorporation of a cleavage...M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale affinity purification of human insulin-like...

  9. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...proteasome machinery to degrade any protein. We are particularly excited about the dTAG system as we can use ...broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules...

  11. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...hormone therapy resulted in 76,000 fewer cases of cardiovascular disease, 126,000 fewer breast cancers, and ... Scientific Impact Are Evoloving Check Out Extracurricular Activities for a Strong Science Career Path...

  12. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...edited embryos proceeded to the blastocyst stage at similar rates to untreated controls. Ma et al. performed...germline editing. Although this paper represents a large step forward in the CRISPR field, it’s important...

  13. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...increased sensitivity, polyclonal antibodies are particularly useful for certain research applications. Many...composition available. If you’re going to need a large quantity of antibody for your experiment, you should...

  14. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ... science and tech get jobs. She is also a very popular speaker and workshop leader. Lauren Celano of Propel...Confident and Successful Industry Professionals”. This larger shop might appeal to some although most scientist...

  15. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...there’s no sense in spending valuable research dollars on a new secondary antibody if you don’t need it... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice...

  16. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...biological questions about the brain. To address this, molecular tools to map neuronal connectivity are widely ... Using these technologies in combination with molecular tools (e.g., optogenetic or chemogenetic receptors...

  17. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...The raw sequencing reads are assembled into a circularized plasmid sequence de novo (without the use of...pipeline. There are regions of plasmids that are particularly difficult to sequence and assemble using the...

  19. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready to use AAV5 viral...

  20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7...
Showing: 661 - 680 of 818 results