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We narrowed to 783 results for: INA

Showing: 341 - 350 of 783 results
  1. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...their best interest to do so, sure, but it also eliminates that feeling of floundering one can have when...also need to talk to Tony from legal, Diana in finance, and Wu in graphic design. You could dread these...career options are expanding. As someone who coordinates cross-team projects at Addgene, I find that my... that led you into science in the first place. Finally, if you still find the idea of not holding a pipette...
  2. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...but some applications have been challenging or inaccessible. The Ting lab recently made two deposits that... Figure 3: A) LOV-Turbo becomes active when illuminated with blue light, catalyzing the biotinylation...that had been trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023.... (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...
  3. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...exposing you to relevant researchers in the field. Finally, you can sound credible and cool when you casually...research papers on these particular themes, for seminal papers on these themes, and also for articles from...citations so that people can go back and read the original reference for the data. For example, we summarized...how big the studies were, and you could find the original paper if you wanted to learn more. Another example...
  4. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ..., homology dependent repair (HR, homologous recombination; SSA, single strand annealing) took place and... with RNAs encoding Cas9 into the oocyte. This original method was developed as the use of CRISPR/Cas9... RNA and therefore there has less chance of contamination with RNase leading to degradation that can set...pups. Taking a toe, a finger, a tail tip, or a combination of these will allow you to determine which pup...
  5. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...circuit, while a nose-poke at a separate hole (inactive hole) does not. If activation of the circuit is...unwanted phenomenon or it could reveal something fascinating. Behavior is based primarily on careful observation...experimenting and hopefully optogenetics will help illuminate (is that pun worn out yet?) your research. Derek...PMCID: PMC4165888. 6.Witten, Ilana B., et al. "Recombinase-driver rat lines: tools, techniques, and optogenetic...
  6. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ... practice, the term "promoter" describes the combination of the promoter (RNA polymerase binding site)...synthetic promoters, which typically include some combination of other promoter elements, and tend to be more...expression mRNA Mammalian promoter from phosphoglycerate kinase gene.  Constitutive  Widespread expression, but...optogenetics mRNA  Ca2+/calmodulin-dependent protein kinase II promoter Specific Used for neuronal/CNS expression...
  7. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...This post was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed...outlined below, the technique can be used as a preliminary validation on your bulk population. This way ...TIDER (Tracking of insertions, deletions, and recombination events). TIDER can screen for specific homology-directed...
  8. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...To acquire these measurements, the cytometer illuminates the passing cell with a laser and detects the...colleagues.   Figure 1: The laser pulse illuminates the cell and is scattered forward at a low angle...represents the time and intensity of the cell’s illumination (see Figure 2). H describes the maximum signal...For our last example plot, I will show you a combination of two stains, in this case through conjugated...
  9. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...the correct size, and purify the DNA fragments. Finally, you simply incubate these three fragments together...loop (as might be anticipated to occur within a terminator sequence), since this would directly compete ...one step with no scar sequences remaining in the final product. The long overlapping region between fragments...different fragments. One way around this is to use a combination of standard sequences with overlap regions, such...
  10. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...engineered proteins responsive to light in combination with retinal) variations designed to give researchers... R., Gunaydin, L. A., Hyun, M., Fenno, L. E., Gradinaru, V., Yizhar, O., & Deisseroth, K. (2012). Principles...
Showing: 341 - 350 of 783 results