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We narrowed to 772 results for: INA

Showing: 341 - 350 of 772 results

  1. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...the correct size, and purify the DNA fragments. Finally, you simply incubate these three fragments together...loop (as might be anticipated to occur within a terminator sequence), since this would directly compete ...one step with no scar sequences remaining in the final product. The long overlapping region between fragments...different fragments. One way around this is to use a combination of standard sequences with overlap regions, such...

  2. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...engineered proteins responsive to light in combination with retinal) variations designed to give researchers... R., Gunaydin, L. A., Hyun, M., Fenno, L. E., Gradinaru, V., Yizhar, O., & Deisseroth, K. (2012). Principles...

  3. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...then be exposed to a lysis buffer to release recombinant proteins directly onto the nitrocellulose for...receptor binding protein hit and can go back to the original plate to identify which phage gene encodes that... 1 M NaCl in order to enhance folding of the recombinant proteins. Optimal salt concentration will vary...colony expressing a functional binding protein was originally located. Now you can grow up your colonies of...

  4. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...American system), in 2009 (at the beginning of the financial recession) I knew obtaining work in scientific...facility, where I tested materials for bacterial contamination. It was a really interesting job at first, everything... are rapidly expanding, as this might not be sustainable. After working at this company for several years...would produce the best outcome for a specific combination of genetic mutations. This is a particular challenge...

  5. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...testing several vectors and packaging cell line combinations, Miller et al. showed that infectious wild-type...are capable of transducing certain quiescent or terminally differentiated cells such as macrophages and ...research due to the possibility that mutation or recombination with human retroviruses could lead to RCL. ...of retrovirus packaging cell lines to avoid recombination leading to helper virus production." Molecular...

  6. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...includes: Michelle, the Senior Scientist who coordinates all kit creation and quality control efforts ...once famously said “I think this is the most extraordinary collection of talent, of human knowledge, that...successfully doled out to multiple people: someone to coordinate, someone to decide, someone to delegate, etc....Next Great Discovery is exactly that: a myth. The original Dr. Frankenstein from Mary Shelley’s novel worked...

  7. Help Us Name Blugene’s New Virus Friend

    Type
    Blog Post
    Published
    Jan. 7, 2020, 1:55 p.m.
    ... contest is now closed. Stay tuned to see the finalists and vote for your favorite! Our mascot who has...neutral name. The Addgenies will choose up to four finalists and we’ll ask you to vote for your favorite name...We hope you submit your best ideas because the finalists will receive some newly designed Addgene swag....

  8. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...Addgene. Boston, MA, USA 42.3601°N 71.0589°W – Alina Chan The job of scientists to observe how things... and generation is in the veracity of its work. Alina Chan is a postdoctoral researcher at Harvard Medical...saying that climate change is a hoax. This has culminated in the current President holding and campaigning...interactions, and science education. Photo cred Alina Chan. Seattle, Washington, USA 47.6062°N 122.3321...

  9. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...structures are more stable and local movements predominate (e.g. a basal progenitor cell migrating to the...fluorescent proteins (Salomonnson et al. 2012). Finally, it is worthwhile to test the photostability of...used to study B and T cell dynamics within the germinal center of murine lymph nodes. However, one must...marking and tracking cells is the the Cre/lox recombination system. A researcher can flank fluorescent proteins...

  10. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...and important details that you won’t find in the final publication and we have already collected over 100...accelerate your research. Sincerely, Melina & The Addgene Team ... Melina Fan...
Showing: 341 - 350 of 772 results