We narrowed to 851 results for: CAL

...could be used in high throughput screening for agrochemical compounds that have the potential to regulate...and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments...

...any DNA editing functionality Collecting preclinical and clinical data about a disease or therapeutic Side-by-side...

...virtual face-to-face meetings as opposed to phone calls most closely replicates how you’d interact with ...investigate my protein of interest in a French clinical cohort, neither of which I would have been able...

...host range and copy number of the plasmid) and typically include a gene that is advantageous for survival...Plasmids 101: Luciferase Monitor the level of a chemical in a particular environment Produce enzymes that...

...complements the auxotrophy (i.e., synthesizes the critical amino acid). Many common E. coli strains used ...its toxic effect. Negative selection is sometimes called counterselection.  Toxin-antitoxin systems One ...

...capsid other than AAV2. This production strategy is called cross-packaging.  Capsid (cap) gene The cap gene... of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248–265 . https://doi.org/10.1016...

...plasmids through our repository since we know biological resource centers, including Addgene, positively.... “Addgene has made it possible to eliminate geographical restrictions in life science research." Claim...

...blogosphere is to read and comment on their blogs. 5)   Local science museums are a great place to find opportunities...Science Communication Program at the University of California, Santa Cruz, has been turning scientists into...

...How else can antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt ...cloning. In these instances, the cloning vector typically has two separate resistance cassettes and your...

...Randolph-Habecker J (2013). A Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free...Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical technique for demonstration of specific binding...

...distribution. I don’t have space in this blog to call out every Addgene Team because they’re all doing...requests for scientists doing almost every type of biomedical science research. Our work accelerates and enhances...

... sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ITRs due to their secondary...of lox sites which are part of a dual lox system called FLEX or DIO. Lox sites are recognized by the recombinase...

...(CellTagD13), which is when the iEP phenotype typically emerges. Every three days, a sample of cells was...conversion, but CellTag could be used to study other biological processes where cells are undergoing identity...

...serotypes are equal; while some serotypes are typically high yielding, for others, such as AAV2, low yields... of adeno-associated virus/phage." Journal of Biological Chemistry (2012): jbc-M112. PubMed PMID: 22915587...

...However, now that we are all so good with video calls, how about trying your hand at a video presentation...the board of a professional society, solved a technical problem, had an alter ego as a mad scientist for...

...analyzing the gene’s function. The process is shown graphically in the following figure, in which we add EcoRI...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...

...Find plasmids from this publication at Addgene. Scalable and versatile genome editing using linear DNAs...Caenorhabditis elegans. Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause MW, Seydoux...

...type; however, their usage has been limited by technical problems. For instance, it’s much more difficult...presence of the CPP may alter protein function or localization. Thus, the usefulness of CPPs must be evaluated...

... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...mentoring meetings a priority. Schedule meetings or calls regularly and don’t cancel, so that you drive progress...

...developed a web-based, career-planning platform called the Individual Development Plan (IDP) and uses ... Outreach activities (e.g. Volunteer at a local high school science class: Science Club for Girls...