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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 817 results for: LARS

Showing: 681 - 700 of 817 results

  1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7...

  2. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...up in this book. Some of these issues are not particularly new. Chapter 2, “The Professional Scientist,... process and the marginalization of science, particularly public health and environmental science, by ...

  3. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150...ease of use, droplet digital PCR is becoming a popular option for PCR experiments.  References Furuta-Hanawa...

  4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...RNPs are capable of cleaving genomic targets with similar efficiency as compared to plasmid-based expression... homology directed repair (HDR), and generating large genomic deletions. What are the advantages of Cas9...

  5. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ... as the central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate...difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ...

  7. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...   Indirect ELISAs The indirect ELISA process is similar to that of a direct ELISA, but the indirect method... drawbacks of the competitive ELISA method are similar to those of the direct method. Namely, since the...

  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...infections, we are treating microbes with compounds similar to those they have been battling for centuries.... that will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...characterization of diverse class 2 CRISPR-Cas systems." Molecular cell 60.3 (2015): 385-397. PubMed PMID: 26593719...

  11. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... match their original description. The oldest, largest, and most diverse of these repositories is the ...out on low passage cells and this means you can regularly discard the cells you are working with because...

  12. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...undergo homology-directed repair (HDR), a distinct cellular DNA repair pathway. Accomplishing this usually... iPSC-based models of neurodegenerative and neurovascular diseases. He worked on the described project...

  13. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...both groups on a western blot and look for the molecular weight shifts indicative of tau phosphorylation...against a protein standard (P) to check for tau molecular weight shifts. A negative control is a sample...

  14. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ... looking at a process-oriented team serving a particular function. Project team A Project Team is assembled...an excuse. Every psych test I’ve ever taken has declared me an introvert, and I identify as one.  But some...

  15. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    [email protected]. These deposits are considered large deposits and are exceptions to our standard workflow...your plasmid is designed to allow you to produce large amounts of your insert in E. coli, then this is ...

  16. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

  17. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ... to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On the fourth day of CRISPR, ...

  18. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...Enhanced prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th ...

  19. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ... technologies are going? What is the next hot molecular biology technology? Have you deposited your plasmids...

  20. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ... 101: Una herramienta de edición  Addgene has a large international audience, including working with distributors...
Showing: 681 - 700 of 817 results