We narrowed to 708 results for: MAK;
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TypeBlog PostPublishedSept. 1, 2020, 1:15 p.m....Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging capacity...
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CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m..... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog PostPublishedDec. 6, 2022, 2:15 p.m....iGECI and NIR-GECO2G, and its small size (~1500 bp) makes iGECInano suitable for AAV packaging. Find iGECInano... -
Plasmids 101: Biotinylation
TypeBlog PostPublishedNov. 15, 2018, 1:50 p.m....exposed side chain that can be easily manipulated to make reactive species without affecting the avidin binding... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog PostPublishedMay 5, 2020, 1:15 p.m....relies on its activity on the target regions thus making it an affinity-based method. Due to the mismatch... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....allowing for stable expression of the transgene. This makes IDLVs an attractive choice for certain types of ... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog PostPublishedApril 8, 2025, 1:15 p.m....controls So how can you address these errors without making incorrect assumptions? The answer depends on the... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....gating uses the same color for different markers, making it easy to gate on the negative cell population... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....position. The “sloppy” PCR reaction is created by making some or all of the following modifications: 1) ... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic mouse lines... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....model than in latent HIV-1 infection, which would make it easier for an “escape” virus to propagate and... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m....adds a repressible element to this binary system (making this a repressible binary system). The GAL80 repressor... -
A Control for All Seasons
TypeBlog PostPublishedOct. 5, 2023, 1:15 p.m.... high degree of variability in the process could make it difficult to obtain meaningful results. Biological... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog PostPublishedJuly 28, 2016, 2:30 p.m....register at a time. If a particular application makes it difficult to isolate single registers or if it... -
Plasmids 101: Restriction Cloning
TypeBlog PostPublishedFeb. 18, 2016, 3:42 p.m....overhangs that allow them to bind. Ligase is used to make bonds between the insert and backbone covalent. ... -
Finding Your Perfect Job After University
TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m....Addgene’s Website and travel to meet scientists, making my job different every day. I love the freedom ... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....studies, the high gene copy number found in plants makes traditional knockouts unattractive from a practical... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m.... friendly neighborhood biostatistician happy by making your statistical analysis plan when you design ... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....selective expression of opsin genes in targeted cells makes it possible to control neuronal activity with a ... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog PostPublishedAug. 5, 2025, 1:15 p.m....-antibody interactions in a plate-based format, making it suitable for comparing relative affinities across...