We narrowed to 729 results for: NSI;

1. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   Published

   May 30, 2023, 1:15 p.m.

   ...and in vivo laboratory experiments ranging from transient gene expression in a particular cell type to CRISPR...

2. Transferable Skills Guide: Public Speaking

   Type

   Blog Post

   Published

   April 20, 2023, 4 p.m.

   ...completely normal to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I'...

3. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   Published

   Oct. 20, 2022, 1:15 p.m.

   ...oxygen. Other useful backbone modifications to consider include amide linkages, unlocked nucleic acids...

4. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   ...complementarity to the gRNA, they have a higher propensity to reform double helices and decrease Cas9 cutting...

5. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   Published

   Oct. 20, 2015, 2:30 p.m.

   ... to train our young scientists for futures both inside and outside of academic research (4). There are...

6. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...assembling the toolbox needed for anyone to begin considering genetic engineering projects in a very big way...

7. Hot Plasmids - October 2020

   Type

   Blog Post

   Published

   Oct. 13, 2020, 1:15 p.m.

   ...myeloid leukemia and saw that expression of H2B-FT consistently predicted the cell cycle speed across these ...

8. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

   Type

   Blog Post

   Published

   July 23, 2020, 1:20 p.m.

   ...activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity to be ...

9. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

   Type

   Blog Post

   Published

   May 7, 2020, 1:15 p.m.

   ... starting point for a new generation of ABEs), consists of 3 components:  a deaminase (TadA, originally...

10. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ...Arg-mutant proteins in the lab, clinicians might consider prescribing GdnHCl to patients suffering from ...

11. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...functions of key regeneration-related genes in four inexpensive and commercially available planarians: Girardia...

12. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...full-time graduate course, UWE Bristol offers an intensive 4-day Masterclass in science communication. The...

13. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...cells are behaving normally can be prohibitively expensive for many labs. We’ve created and tested plasmids...

14. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...us at [email protected]. These deposits are considered large deposits and are exceptions to our standard...

15. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...Off-target effects are also important safety considerations for all CRISPR therapies, but especially concerning...

16. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    Published

    Aug. 1, 2023, 1:15 p.m.

    ...Thomas C. Evans, J., & Lohman, G. J. S. (2018). Comprehensive Profiling of Four Base Overhang Ligation Fidelity...

17. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    Published

    Nov. 14, 2024, 2:15 p.m.

    ... a number of different options. By carefully considering your ncRNA of interest, you should be able to...

18. Plasmids 101: Modular Cloning

    Type

    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ...Scripps Research, where he works on genetic code expansion.  References and Resources References Weber E...

19. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    Published

    Aug. 30, 2016, 2:30 p.m.

    ...of toehold switch-based RNA sensors within an extensive infrastructure of molecular and electronic companion...

20. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...the target sequence (bases 19-23). Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver...