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  1. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...increasingly seeking us out on their own to share reagents. I love the way scientists starting their own ...scientists who request from us but also by high school students and industry scientists as a universal resource...

  2. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...not cancer cell populations resistant to drug treatments arise during treatment or are preexisting in ...really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative...

  4. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...-species staining protocols that use antibody fragments to block the endogenous immunoglobulins and reduce...complex with a variety of proteins interacting. Experiments tend to be equally complex with users trying ...

  5. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...Antibodies are used in many different experiments that require scientists to detect proteins in their...visualization. The indirect ELISA is used for similar experiments as the direct ELISA, the only difference being...

  7. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...DNA as opposed to in live bacteria. For these shipments, we’ve needed to find a way to purify DNA from... tips and tricks, be sure to share them in the comments section.   Additional Resources on the Addgene...

  8. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...more complex and the presence of helper virus contaminants have slowed the use of this system. New and ...purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation to separate ...

  9. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ... al., 2013). The study found that at early time points, most of the projections to these new neurons came...from nearby neurons. It wasn’t until later time points that longer-range connections were seen. This study...

  10. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ... best strategies and protocols for different experiments.  In this blog post let's take a look at some...lab in 2015 adapted the AID system discovered in plants to conditionally deplete proteins of interest in...

  11. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...phenotype and helps improve the reproducibility of experiments. Inbred mice are particularly useful for understanding...recessive traits and their impact on diseases or treatments, but are not useful for genetic engineering. ...

  12. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...format depending on the particular style and requirements of the new Board Members. In some instances ...when it is so important for postdocs and graduate students to think about their career options, gaining transferable...

  16. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...the conference Review your favorite conference moments on social media and if there was something you ... with people you don’t know or attending social events. I like to set goals for myself like meet ten new...

  17. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...marker HSV-tk is used to select against random recombinants.   Design your targeting construct. For...participating in development or other physiopathological events to which the chosen promoter responds. You can ...

  18. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Because it is made at the same time as the other components of the vector particle, some of the RT protein... Addgene blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors...

  19. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...While challenges remain in the delivery of these agents, CRISPR antimicrobials could become our newest ... both favors antibiotic sensitive bacteria and prevents sensitive bacteria from acquiring resistance genes...

  20. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...and me. To that end, here are my personal 10 commandments for making it all work – most of the time. #1...transportation, call a cab and remember their own school assignments, the better. In my opinion, a level of independence...
Showing: 681 - 700 of 840 results